Abstract
Volatile anesthetics modulate a variety of physiological and pathophysiological responses including hypoxic responses. Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including erythropoiesis, angiogenesis, and glycolysis. We demonstrate for the first time that the volatile anesthetic halothane blocks HIF-1 activity and downstream target gene expressions induced by hypoxia in the human hepatoma-derived cell line, Hep3B. Halothane reversibly blocks hypoxia-induced HIF-1α protein accumulation and transcriptional activity at clinically relevant doses.
Original language | English (US) |
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Pages (from-to) | 225-229 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 509 |
Issue number | 2 |
DOIs | |
State | Published - Dec 7 2001 |
Keywords
- Gene expression
- Halothane
- Hypoxia-inducible factor 1
- Volatile anesthetic
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology