Reversible inhibition of hypoxia-inducible factor 1 activation by exposure of hypoxic cells to the volatile anesthetic halothane

Tatsuya Itoh, Tsunehisa Namba, Kazuhiko Fukuda, Gregg L. Semenza, Kiichi Hirota

Research output: Contribution to journalArticlepeer-review

Abstract

Volatile anesthetics modulate a variety of physiological and pathophysiological responses including hypoxic responses. Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including erythropoiesis, angiogenesis, and glycolysis. We demonstrate for the first time that the volatile anesthetic halothane blocks HIF-1 activity and downstream target gene expressions induced by hypoxia in the human hepatoma-derived cell line, Hep3B. Halothane reversibly blocks hypoxia-induced HIF-1α protein accumulation and transcriptional activity at clinically relevant doses.

Original languageEnglish (US)
Pages (from-to)225-229
Number of pages5
JournalFEBS Letters
Volume509
Issue number2
DOIs
StatePublished - Dec 7 2001

Keywords

  • Gene expression
  • Halothane
  • Hypoxia-inducible factor 1
  • Volatile anesthetic

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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