Reversible (G0) and nonreadily reversible (Q) noncycling cells in human peripheral blood - Immunological, structural, and biological characterization

S. Abraham; E. Vonderheid; S. Zietz; F. M. Kendall; C. Nicolini

doi:10.1007/BF02785099

Reversible (G0) and nonreadily reversible (Q) noncycling cells in human peripheral blood - Immunological, structural, and biological characterization

S. Abraham, E. Vonderheid, S. Zietz, F. M. Kendall, C. Nicolini

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

PHA-stimulated human lymphoctes (normal-resting-proliferating) at 0, 24, 48, 72, and 144 h were studied with Acridine Orange (AO) staining. By viable cell sorting, by subsequent subculturing, and by use of biochemical, biophysical, and immunological assays, not only have the G₀ resting and G₁ (cycling) cell cycle phases been objectively characterized, but a separate subpopulation of quiescent cells that are functionally viable and deeply committed to nonproliferation, the Q cells, has been identified. Multiparameter cytofluorimetric analysis, methyl¹⁴C-thymidine incorporation, automated image analysis, and mitogen stimulation studies have shown that the "Q" cell, compared to the "G₀" resting but easily recruitable cell, exhibits quite lower red and green AO emission, possesses 2c to 4c DNA content (rather than only 2c), has a higher average optical density, and is either nonrecruitable or recruitable-with-difficulty in PHA-stimulated lymphocyte cultures.

Original language	English (US)
Pages (from-to)	353-371
Number of pages	19
Journal	Cell Biophysics
Volume	2
Issue number	4
DOIs	https://doi.org/10.1007/BF02785099
State	Published - Dec 1980
Externally published	Yes

Keywords

automated image analysis, of human lymphocytes
blood, G, and Q cells in
cell sorting, of G, G, and Q cells
cytofluorometry of noncycling lymphocytes
G cells in peripheral blood
human blood, G and Q cells in
lymphocytes, characterization of
methylC-thymidine incorporation, in human lymphocytes
mitogen-stimulated lymphocytes, characterization of
nonreadily reversible cells in blood
optical density, of human lymphocytes
Q cells in peripheral blood
Reversible noncycling cells in blood

ASJC Scopus subject areas

Cell Biology
Biophysics

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10.1007/BF02785099

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title = "Reversible (G0) and nonreadily reversible (Q) noncycling cells in human peripheral blood - Immunological, structural, and biological characterization",

abstract = "PHA-stimulated human lymphoctes (normal-resting-proliferating) at 0, 24, 48, 72, and 144 h were studied with Acridine Orange (AO) staining. By viable cell sorting, by subsequent subculturing, and by use of biochemical, biophysical, and immunological assays, not only have the G0 resting and G1 (cycling) cell cycle phases been objectively characterized, but a separate subpopulation of quiescent cells that are functionally viable and deeply committed to nonproliferation, the Q cells, has been identified. Multiparameter cytofluorimetric analysis, methyl14C-thymidine incorporation, automated image analysis, and mitogen stimulation studies have shown that the {"}Q{"} cell, compared to the {"}G0{"} resting but easily recruitable cell, exhibits quite lower red and green AO emission, possesses 2c to 4c DNA content (rather than only 2c), has a higher average optical density, and is either nonrecruitable or recruitable-with-difficulty in PHA-stimulated lymphocyte cultures.",

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author = "S. Abraham and E. Vonderheid and S. Zietz and Kendall, {F. M.} and C. Nicolini",

year = "1980",

month = dec,

doi = "10.1007/BF02785099",

language = "English (US)",

volume = "2",

pages = "353--371",

journal = "Cell Biophysics",

issn = "0163-4992",

publisher = "Humana Press",

number = "4",

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TY - JOUR

T1 - Reversible (G0) and nonreadily reversible (Q) noncycling cells in human peripheral blood - Immunological, structural, and biological characterization

AU - Abraham, S.

AU - Vonderheid, E.

AU - Zietz, S.

AU - Kendall, F. M.

AU - Nicolini, C.

PY - 1980/12

Y1 - 1980/12

N2 - PHA-stimulated human lymphoctes (normal-resting-proliferating) at 0, 24, 48, 72, and 144 h were studied with Acridine Orange (AO) staining. By viable cell sorting, by subsequent subculturing, and by use of biochemical, biophysical, and immunological assays, not only have the G0 resting and G1 (cycling) cell cycle phases been objectively characterized, but a separate subpopulation of quiescent cells that are functionally viable and deeply committed to nonproliferation, the Q cells, has been identified. Multiparameter cytofluorimetric analysis, methyl14C-thymidine incorporation, automated image analysis, and mitogen stimulation studies have shown that the "Q" cell, compared to the "G0" resting but easily recruitable cell, exhibits quite lower red and green AO emission, possesses 2c to 4c DNA content (rather than only 2c), has a higher average optical density, and is either nonrecruitable or recruitable-with-difficulty in PHA-stimulated lymphocyte cultures.

AB - PHA-stimulated human lymphoctes (normal-resting-proliferating) at 0, 24, 48, 72, and 144 h were studied with Acridine Orange (AO) staining. By viable cell sorting, by subsequent subculturing, and by use of biochemical, biophysical, and immunological assays, not only have the G0 resting and G1 (cycling) cell cycle phases been objectively characterized, but a separate subpopulation of quiescent cells that are functionally viable and deeply committed to nonproliferation, the Q cells, has been identified. Multiparameter cytofluorimetric analysis, methyl14C-thymidine incorporation, automated image analysis, and mitogen stimulation studies have shown that the "Q" cell, compared to the "G0" resting but easily recruitable cell, exhibits quite lower red and green AO emission, possesses 2c to 4c DNA content (rather than only 2c), has a higher average optical density, and is either nonrecruitable or recruitable-with-difficulty in PHA-stimulated lymphocyte cultures.

KW - automated image analysis, of human lymphocytes

KW - blood, G, and Q cells in

KW - cell sorting, of G, G, and Q cells

KW - cytofluorometry of noncycling lymphocytes

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KW - lymphocytes, characterization of

KW - methylC-thymidine incorporation, in human lymphocytes

KW - mitogen-stimulated lymphocytes, characterization of

KW - nonreadily reversible cells in blood

KW - optical density, of human lymphocytes

KW - Q cells in peripheral blood

KW - Reversible noncycling cells in blood

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Reversible (G0) and nonreadily reversible (Q) noncycling cells in human peripheral blood - Immunological, structural, and biological characterization

Abstract

Keywords

ASJC Scopus subject areas

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