Reverse transcriptase reads through a 2′-5′ linkage and a 2′-thiphosphate in a template

John R. Lorsch, David P. Bartel, Jack W. Szostak

Research output: Contribution to journalArticlepeer-review

Abstract

Avian myeloblastosis virus and Maloney murlne leukemia virus RNase H- reverse transcriptases pause when they encounter a 2′-5′ linkage or a 2′-thiophosphate In their template RNAs, but eventually read through these backbone modifications. Both reverse transcriptases pause after the 2′-5′ linkage but before the 2′-thlophosphate. These results suggest that in the absence of precise information concerning the behavior of a given reverse transcriptase with respect to a particular lesion or modification, caution should be exercised in the interpretation of primer extension data that is being used to determine the existence of, or map the position of, a crosslink, site of chemical modification or non-standard linkage in an RNA template.

Original languageEnglish (US)
Pages (from-to)2811-2814
Number of pages4
JournalNucleic acids research
Volume23
Issue number15
DOIs
StatePublished - Aug 11 1995

ASJC Scopus subject areas

  • Genetics

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