Reverse transcriptase dideoxy sequencing of RNA

Sarah E. Walker, Jon Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

During the course of studying the structure and function of RNA molecules and the proteins that they may encode, as well as studies of the expression of a given gene, there often arises a need to learn the nucleotide sequence of an RNA molecule. While the sequence of a particular RNA is ordinarily encompassed within a corresponding DNA sequence, RNA sequencing lends the advantage of giving the transcription initiation and termination sequences, and the sites where modifications from the original DNA sequence occur.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc.
Pages347-359
Number of pages13
ISBN (Print)9780124200371
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume530
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Anneal primer
  • Label primer
  • Primer extension and chain termination
  • Reverse transcriptase dideoxy sequencing
  • UREA-PAGE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Reverse transcriptase dideoxy sequencing of RNA'. Together they form a unique fingerprint.

Cite this