Reverse transcriptase dideoxy sequencing of RNA

Sarah E. Walker, Jon Lorsch

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

During the course of studying the structure and function of RNA molecules and the proteins that they may encode, as well as studies of the expression of a given gene, there often arises a need to learn the nucleotide sequence of an RNA molecule. While the sequence of a particular RNA is ordinarily encompassed within a corresponding DNA sequence, RNA sequencing lends the advantage of giving the transcription initiation and termination sequences, and the sites where modifications from the original DNA sequence occur.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
Pages347-359
Number of pages13
Volume530
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume530
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

RNA Sequence Analysis
RNA-Directed DNA Polymerase
RNA
DNA sequences
Molecules
Transcription
Nucleotides
Genes
Proteins

Keywords

  • Anneal primer
  • Label primer
  • Primer extension and chain termination
  • Reverse transcriptase dideoxy sequencing
  • UREA-PAGE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Walker, S. E., & Lorsch, J. (2013). Reverse transcriptase dideoxy sequencing of RNA. In Methods in Enzymology (Vol. 530, pp. 347-359). (Methods in Enzymology; Vol. 530). https://doi.org/10.1016/B978-0-12-420037-1.00020-8

Reverse transcriptase dideoxy sequencing of RNA. / Walker, Sarah E.; Lorsch, Jon.

Methods in Enzymology. Vol. 530 2013. p. 347-359 (Methods in Enzymology; Vol. 530).

Research output: Chapter in Book/Report/Conference proceedingChapter

Walker, SE & Lorsch, J 2013, Reverse transcriptase dideoxy sequencing of RNA. in Methods in Enzymology. vol. 530, Methods in Enzymology, vol. 530, pp. 347-359. https://doi.org/10.1016/B978-0-12-420037-1.00020-8
Walker SE, Lorsch J. Reverse transcriptase dideoxy sequencing of RNA. In Methods in Enzymology. Vol. 530. 2013. p. 347-359. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420037-1.00020-8
Walker, Sarah E. ; Lorsch, Jon. / Reverse transcriptase dideoxy sequencing of RNA. Methods in Enzymology. Vol. 530 2013. pp. 347-359 (Methods in Enzymology).
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