Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3

Arjan J. Groot; Mohamed El Khattabi; Norman Sachs; Petra van der Groep; Elsken van der Wall; Paul J. van Diest; Arnoud Sonnenberg; C. Theo Verrips; Marc Vooijs

doi:10.1016/j.molimm.2009.03.002

Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3

Arjan J. Groot, Mohamed El Khattabi, Norman Sachs, Petra van der Groep, Elsken van der Wall, Paul J. van Diest, Arnoud Sonnenberg, C. Theo Verrips, Marc Vooijs

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin α3β1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.

Original language	English (US)
Pages (from-to)	2022-2028
Number of pages	7
Journal	Molecular Immunology
Volume	46
Issue number	10
DOIs	https://doi.org/10.1016/j.molimm.2009.03.002
State	Published - Jun 2009
Externally published	Yes

Keywords

Anti adhesive VHH
Integrin
Phage-display
Surface marker
VLA-3

ASJC Scopus subject areas

Immunology
Molecular Biology

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10.1016/j.molimm.2009.03.002

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@article{907fe42fd8234ef088e2beda08c4fd59,

title = "Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3",

abstract = "Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin α3β1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.",

keywords = "Anti adhesive VHH, Integrin, Phage-display, Surface marker, VLA-3",

author = "Groot, {Arjan J.} and Khattabi, {Mohamed El} and Norman Sachs and {van der Groep}, Petra and {van der Wall}, Elsken and {van Diest}, {Paul J.} and Arnoud Sonnenberg and Verrips, {C. Theo} and Marc Vooijs",

note = "Funding Information: The authors would like to acknowledge M. Paalanen, D. van Breevoort and M. van Gent (University of Utrecht) for technical assistance with phage-display, I. Verlaan (UMCU) for help with immunoprecipitations and T. van Laar (UMCU) for help with the FACS analysis. This work was supported by The Dutch Cancer Society (UU2003-2825), and by the 2nd AEGON international Scholarschip in Oncology.",

year = "2009",

month = jun,

doi = "10.1016/j.molimm.2009.03.002",

language = "English (US)",

volume = "46",

pages = "2022--2028",

journal = "Molecular Immunology",

issn = "0161-5890",

publisher = "Elsevier Limited",

number = "10",

}

TY - JOUR

T1 - Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3

AU - Groot, Arjan J.

AU - Khattabi, Mohamed El

AU - Sachs, Norman

AU - van der Groep, Petra

AU - van der Wall, Elsken

AU - van Diest, Paul J.

AU - Sonnenberg, Arnoud

AU - Verrips, C. Theo

AU - Vooijs, Marc

N1 - Funding Information: The authors would like to acknowledge M. Paalanen, D. van Breevoort and M. van Gent (University of Utrecht) for technical assistance with phage-display, I. Verlaan (UMCU) for help with immunoprecipitations and T. van Laar (UMCU) for help with the FACS analysis. This work was supported by The Dutch Cancer Society (UU2003-2825), and by the 2nd AEGON international Scholarschip in Oncology.

PY - 2009/6

Y1 - 2009/6

N2 - Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin α3β1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.

AB - Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin α3β1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.

KW - Anti adhesive VHH

KW - Integrin

KW - Phage-display

KW - Surface marker

KW - VLA-3

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U2 - 10.1016/j.molimm.2009.03.002

DO - 10.1016/j.molimm.2009.03.002

M3 - Article

C2 - 19359042

AN - SCOPUS:67349193615

SN - 0161-5890

VL - 46

SP - 2022

EP - 2028

JO - Molecular Immunology

JF - Molecular Immunology

IS - 10

ER -

Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3

Abstract

Keywords

ASJC Scopus subject areas

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