Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3

Arjan J. Groot, Mohamed El Khattabi, Norman Sachs, Petra van der Groep, Elsken van der Wall, Paul J. van Diest, Arnoud Sonnenberg, C. Theo Verrips, Marc Vooijs

Research output: Contribution to journalArticle

Abstract

Therapeutic approaches aimed at targeting tumor surface markers using monoclonal antibodies provide a powerful strategy in cancer treatment. Here we report selection of single variable domains (VHH) of llama heavy chain antibodies, using a VHH-phage-display library. A reverse proteomic approach was used to identify the cognate proteins recognized by enriched VHH on HeLa cells. One of these VHH bound the integrin α3β1 (VLA-3) and was further characterized. Most interestingly, this VHH could inhibit VLA-3 mediated cell-matrix adhesion. Our approach provides a fast and efficient method to screen for novel cell surface markers on normal and tumor cells that may find diagnostic or therapeutic application in disease management or treatment.

Original languageEnglish (US)
Pages (from-to)2022-2028
Number of pages7
JournalMolecular Immunology
Volume46
Issue number10
DOIs
StatePublished - Jun 1 2009

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Keywords

  • Anti adhesive VHH
  • Integrin
  • Phage-display
  • Surface marker
  • VLA-3

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

Cite this

Groot, A. J., Khattabi, M. E., Sachs, N., van der Groep, P., van der Wall, E., van Diest, P. J., Sonnenberg, A., Verrips, C. T., & Vooijs, M. (2009). Reverse proteomic antibody screening identifies anti adhesive VHH targeting VLA-3. Molecular Immunology, 46(10), 2022-2028. https://doi.org/10.1016/j.molimm.2009.03.002