Reverse phase high-pressure liquid chromatographic separation of retinoids, including retinylphosphate and mannosylretinylphosphate

Pangala V. Bhat, Luigi M. De Luca, Marilyn L. Wind

Research output: Contribution to journalArticlepeer-review

Abstract

Reverse phase hplc was used to separate retinylphosphate mannosylretinylphosphate from other retinoids. These phosphorylated retinoids constituted 12 and 42% of the extracted radioactivity in OEL mouse intestine at 3 and 24 h respectively after the intubation of [1-3H]retinol. A detailed procedure for the chemical synthesis of retinylphosphate is described.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalAnalytical biochemistry
Volume102
Issue number1
DOIs
StatePublished - Feb 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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