Reversal of loss of imprinting in tumor cells by 5-aza-2'-deoxycytidine

Janet M. Barletta, Shirley Rainier, Andrew P Feinberg

Research output: Contribution to journalArticle

Abstract

To determine whether loss of imprinting in cancer might be reversed by altering DNA methylation, we treated tumor cells with 5-aza-2'-deoxycytidine, a specific inhibitor of cytosine DNA methyltransferase. Treated cells showed several significant and reproducible changes. (a) Equal expression of maternal and paternal alleles of insulin-like growth factor 2 switched to predominant expression of a single parental allele. (b) H19 expression was reactivated. (c) Biallelic H19 expression switched to monoallelic expression. (d) Biallelic methylation of H19 switched to preferential allelic methylation. These results imply that abnormally imprinted cells are susceptible to epigenetic modification and that the effect of 5-aza-2'- deoxycytidine on tumor cells with loss of imprinting is not random but specific to one allele.

Original languageEnglish (US)
Pages (from-to)48-50
Number of pages3
JournalCancer Research
Volume57
Issue number1
StatePublished - 1997

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decitabine
Alleles
Methylation
Neoplasms
Cytosine
Methyltransferases
Somatomedins
DNA Methylation
Epigenomics
Mothers
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Reversal of loss of imprinting in tumor cells by 5-aza-2'-deoxycytidine. / Barletta, Janet M.; Rainier, Shirley; Feinberg, Andrew P.

In: Cancer Research, Vol. 57, No. 1, 1997, p. 48-50.

Research output: Contribution to journalArticle

Barletta, Janet M. ; Rainier, Shirley ; Feinberg, Andrew P. / Reversal of loss of imprinting in tumor cells by 5-aza-2'-deoxycytidine. In: Cancer Research. 1997 ; Vol. 57, No. 1. pp. 48-50.
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