Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model

Rani Kunjithapatham, Swathi Karthikeyan, Jean Francois Geschwind, Esther Kieserman, MingDe Lin, De Xue Fu, Shanmugasundara Ganapathy

Research output: Contribution to journalArticle

Abstract

Lack of an in vitro model of metastasis has been a major impediment in understanding the molecular regulation of metastatic processes, and identification of specific therapeutic targets. We have established an in vitro model which displayed the signatures of metastatic phenotype such as migration, invasiveness, chemoresistance and expression of cancer stem-cell markers. This in vitro model was developed by the induction of reversal of multicellular spheroids that were generated by anchorage-independent growth. In vivo data further validated the metastatic phenotype of the in vitro model. Besides delineating the molecular events of metastasis, this model could also improve the screening efficiency of antimetastatic agents.

Original languageEnglish (US)
Article number6816
JournalScientific Reports
Volume4
DOIs
StatePublished - Oct 29 2014

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Cellular Spheroids
Neoplasm Metastasis
Phenotype
Neoplastic Stem Cells
In Vitro Techniques
Growth

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Kunjithapatham, R., Karthikeyan, S., Geschwind, J. F., Kieserman, E., Lin, M., Fu, D. X., & Ganapathy, S. (2014). Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model. Scientific Reports, 4, [6816]. https://doi.org/10.1038/srep06816

Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model. / Kunjithapatham, Rani; Karthikeyan, Swathi; Geschwind, Jean Francois; Kieserman, Esther; Lin, MingDe; Fu, De Xue; Ganapathy, Shanmugasundara.

In: Scientific Reports, Vol. 4, 6816, 29.10.2014.

Research output: Contribution to journalArticle

Kunjithapatham R, Karthikeyan S, Geschwind JF, Kieserman E, Lin M, Fu DX et al. Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model. Scientific Reports. 2014 Oct 29;4. 6816. https://doi.org/10.1038/srep06816
Kunjithapatham, Rani ; Karthikeyan, Swathi ; Geschwind, Jean Francois ; Kieserman, Esther ; Lin, MingDe ; Fu, De Xue ; Ganapathy, Shanmugasundara. / Reversal of anchorage-independent multicellular spheroid into a monolayer mimics a metastatic model. In: Scientific Reports. 2014 ; Vol. 4.
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