Retrovirus-like vectors for Saccharomyces cerevisiae: integration of foreign genes controlled by efficient promoters into yeast chromosomal DNA

E. Jacobs, M. Dewerchin, J. D. Bocke

Research output: Contribution to journalArticlepeer-review

Abstract

Using modified Saccharomyces cerevisiae Ty1 elements located on a 2μ. plasmid, reverse-transcriptase-mediated transposition into yeast chromosomes of expression cassettes containing a foreign gene can be induced. These expression cassettes consist of the yeast ARG3 and CUP1 promoter sequences fused to the Escherichia coli galK structural gene. Expression cassettes as large as 2 kb can be inserted into Ty elements and transposed efficiently to various sites in the yeast genome. A third yeast promoter (from the yeast CAR1 gene) seems to be unsuitable for use in the expression cassette. This may be because it does not allow the transcription run-through necessary for Ty1 transposition. Ways of improving this vector system are discussed, as are its advantages over episomal vector systems.

Original languageEnglish (US)
Pages (from-to)259-269
Number of pages11
JournalGene
Volume67
Issue number2
DOIs
StatePublished - Jul 30 1988

Keywords

  • 2fi plasmid
  • Retrotransposon
  • amplification of genes
  • expression cassette
  • promoter
  • recombinant DNA
  • reverse transcription
  • yeast

ASJC Scopus subject areas

  • Genetics

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