Abstract
Using modified Saccharomyces cerevisiae Ty1 elements located on a 2μ. plasmid, reverse-transcriptase-mediated transposition into yeast chromosomes of expression cassettes containing a foreign gene can be induced. These expression cassettes consist of the yeast ARG3 and CUP1 promoter sequences fused to the Escherichia coli galK structural gene. Expression cassettes as large as 2 kb can be inserted into Ty elements and transposed efficiently to various sites in the yeast genome. A third yeast promoter (from the yeast CAR1 gene) seems to be unsuitable for use in the expression cassette. This may be because it does not allow the transcription run-through necessary for Ty1 transposition. Ways of improving this vector system are discussed, as are its advantages over episomal vector systems.
Original language | English (US) |
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Pages (from-to) | 259-269 |
Number of pages | 11 |
Journal | Gene |
Volume | 67 |
Issue number | 2 |
DOIs | |
State | Published - Jul 30 1988 |
Keywords
- 2fi plasmid
- Retrotransposon
- amplification of genes
- expression cassette
- promoter
- recombinant DNA
- reverse transcription
- yeast
ASJC Scopus subject areas
- Genetics