Retrotransposon R1Bm endonuclease cleaves the target sequence

Qinghua Feng, Gerald Schumann, Jef D. Boeke

Research output: Contribution to journalArticle

Abstract

The R1Bm element, found in the silkworm Bombyx mori, is a member of a group of widely distributed retrotransposons that lack long terminal repeats. Some of these elements are highly sequence-specific and others, like the human L1 sequence, are less so. The majority of R1Bm elements are associated with ribosomal DNA (rDNA). R1Bm inserts into 28S rDNA at a specific sequence; after insertion it is flanked by a specific 14-bp target site duplication of the 28S rDNA. The basis for this sequence specificity is unknown. We show that R1Bm encodes an enzyme related to the endonuclease found in the human L1 retrotransposon and also to the aparinic/apyrimidinic endonucleases. We expressed and purified the enzyme from bacteria and showed that it cleaves in vitro precisely at the positions in rDNA corresponding to the boundaries of the 14-bp target site duplication. We conclude that the function of the retrotransposon endonucleases is to define and cleave target site DNA.

Original languageEnglish (US)
Pages (from-to)2083-2088
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number5
DOIs
StatePublished - Mar 3 1998

Fingerprint

Retroelements
Endonucleases
Ribosomal DNA
Bombyx
Terminal Repeat Sequences
Enzymes
Bacteria
DNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Retrotransposon R1Bm endonuclease cleaves the target sequence. / Feng, Qinghua; Schumann, Gerald; Boeke, Jef D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 5, 03.03.1998, p. 2083-2088.

Research output: Contribution to journalArticle

Feng, Qinghua ; Schumann, Gerald ; Boeke, Jef D. / Retrotransposon R1Bm endonuclease cleaves the target sequence. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 5. pp. 2083-2088.
@article{e0d33136a28f46a692b0bce010d06bab,
title = "Retrotransposon R1Bm endonuclease cleaves the target sequence",
abstract = "The R1Bm element, found in the silkworm Bombyx mori, is a member of a group of widely distributed retrotransposons that lack long terminal repeats. Some of these elements are highly sequence-specific and others, like the human L1 sequence, are less so. The majority of R1Bm elements are associated with ribosomal DNA (rDNA). R1Bm inserts into 28S rDNA at a specific sequence; after insertion it is flanked by a specific 14-bp target site duplication of the 28S rDNA. The basis for this sequence specificity is unknown. We show that R1Bm encodes an enzyme related to the endonuclease found in the human L1 retrotransposon and also to the aparinic/apyrimidinic endonucleases. We expressed and purified the enzyme from bacteria and showed that it cleaves in vitro precisely at the positions in rDNA corresponding to the boundaries of the 14-bp target site duplication. We conclude that the function of the retrotransposon endonucleases is to define and cleave target site DNA.",
author = "Qinghua Feng and Gerald Schumann and Boeke, {Jef D.}",
year = "1998",
month = "3",
day = "3",
doi = "10.1073/pnas.95.5.2083",
language = "English (US)",
volume = "95",
pages = "2083--2088",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "5",

}

TY - JOUR

T1 - Retrotransposon R1Bm endonuclease cleaves the target sequence

AU - Feng, Qinghua

AU - Schumann, Gerald

AU - Boeke, Jef D.

PY - 1998/3/3

Y1 - 1998/3/3

N2 - The R1Bm element, found in the silkworm Bombyx mori, is a member of a group of widely distributed retrotransposons that lack long terminal repeats. Some of these elements are highly sequence-specific and others, like the human L1 sequence, are less so. The majority of R1Bm elements are associated with ribosomal DNA (rDNA). R1Bm inserts into 28S rDNA at a specific sequence; after insertion it is flanked by a specific 14-bp target site duplication of the 28S rDNA. The basis for this sequence specificity is unknown. We show that R1Bm encodes an enzyme related to the endonuclease found in the human L1 retrotransposon and also to the aparinic/apyrimidinic endonucleases. We expressed and purified the enzyme from bacteria and showed that it cleaves in vitro precisely at the positions in rDNA corresponding to the boundaries of the 14-bp target site duplication. We conclude that the function of the retrotransposon endonucleases is to define and cleave target site DNA.

AB - The R1Bm element, found in the silkworm Bombyx mori, is a member of a group of widely distributed retrotransposons that lack long terminal repeats. Some of these elements are highly sequence-specific and others, like the human L1 sequence, are less so. The majority of R1Bm elements are associated with ribosomal DNA (rDNA). R1Bm inserts into 28S rDNA at a specific sequence; after insertion it is flanked by a specific 14-bp target site duplication of the 28S rDNA. The basis for this sequence specificity is unknown. We show that R1Bm encodes an enzyme related to the endonuclease found in the human L1 retrotransposon and also to the aparinic/apyrimidinic endonucleases. We expressed and purified the enzyme from bacteria and showed that it cleaves in vitro precisely at the positions in rDNA corresponding to the boundaries of the 14-bp target site duplication. We conclude that the function of the retrotransposon endonucleases is to define and cleave target site DNA.

UR - http://www.scopus.com/inward/record.url?scp=0032478313&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032478313&partnerID=8YFLogxK

U2 - 10.1073/pnas.95.5.2083

DO - 10.1073/pnas.95.5.2083

M3 - Article

VL - 95

SP - 2083

EP - 2088

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 5

ER -