Retinoid-regulated expression of BCL-2 and tissue transglutaminase during the differentiation and apoptosis of human myeloid leukemia (HL-60) cells

László Nagy, Vilmos A. Thomázy, Roshantha A.S. Chandraratna, Richard A. Heyman, Peter J.A. Davies

Research output: Contribution to journalArticle

Abstract

Retinoids induce terminal differentiation and subsequent apoptosis in the human myeloid leukemia (HL-60) cell line. We have previously shown that in HL-60 cells, ligand activation of retinoic acid receptors (RARs) is sufficient to induce differentiation but ligand activation of retinoid X receptors (RXRs) is necessary for the retinoid-induced apoptosis of these cells. In the present studies we have characterized the effect of retinoids on the expression of two apoptosis-linked gene products, BCL-2 and tissue transglutaminase. BCL-2 is a membrane-associated protein whose expression has been linked to the suppression of apoptosis in many cells. Tissue transglutaminase is a protein cross-linking enzyme that accumulates in many cells undergoing apoptotic cell death. Our data suggest that ligand activation of RARs in HL-60 cells results in a global suppression of BCL-2 expression whereas ligand activation of both RARs and RXRs triggers the selective accumulation of tissue transglutaminase in the apoptotic HL-60 cells.

Original languageEnglish (US)
Pages (from-to)499-505
Number of pages7
JournalLeukemia Research
Volume20
Issue number6
DOIs
StatePublished - Jun 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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