Retinoid processing in cone and Müller cell lines

Yogita Kanan, Anne Kasus-Jacobi, Gennadiy Moiseyev, Kjell Sawyer, Jian Xing Ma, Muayyad R. Al-Ubaidi

Research output: Contribution to journalArticlepeer-review

Abstract

To determine whether cones and Müller cells in the rod dominated retina cooperate to regenerate the 11-cis retinal chromophore via the retinoid cycle, two cell lines from the rod dominated retinas of Murine were used for this study: 661W, a mouse cell line derived from cones, and rMC-1, a rat Müller cell line. Retinoid cycle enzymes were analyzed by RT-PCR, and their catalytic activity was detected by incubation with retinoids and analyzed by HPLC. We found that 661W cells are capable of reducing all-trans retinal to all-trans retinol due to the presence of multiple dehydrogenases and to generate minor amounts of retinyl-ester. The rMC-1 cells take up all-trans retinol and oxidize it to all-trans retinal or esterify it to retinyl-ester, but are incapable of isomerizing all-trans retinoids to 11-cis retinoids. This could be a reflection of lack of necessary activities in Müller cells in vivo, which suggests that Müller cells do not contribute to retinoid cycling by regenerating 11-cis retinoids. Alternatively, this could be due to the potential that rMC-1, as a transformed cell line, has stopped expressing the proteins needed for the regeneration of 11-cis retinoids.

Original languageEnglish (US)
Pages (from-to)344-354
Number of pages11
JournalExperimental eye research
Volume86
Issue number2
DOIs
StatePublished - Feb 2008
Externally publishedYes

Keywords

  • cone photoreceptors
  • retinal cell culture
  • retinoid recycling
  • retinoids

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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