TY - JOUR
T1 - Retinoid processing in cone and Müller cell lines
AU - Kanan, Yogita
AU - Kasus-Jacobi, Anne
AU - Moiseyev, Gennadiy
AU - Sawyer, Kjell
AU - Ma, Jian Xing
AU - Al-Ubaidi, Muayyad R.
N1 - Funding Information:
The project described was supported by grants from the National Center For Research Resources (P20RR017703) and the National Eye Institute (P30EY12190 and R01EY EY14052 (M.R.A.) and R01EY012231 and EY015650 (J.X.M.)). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center For Research Resources, the National Eye Institute, or the National Institutes of Health. Further support was provided by the Knights Templar Eye Foundation, Inc. (Y.K.). The authors are grateful to Drs. Anand Swaroop (Kellogg Eye Center, Ann Arbor, MI) for providing the Nrl −/− mice and Dr. Rosalie Crouch (Medical University of South Carolina, Charleston, SC) for providing 11- cis retinol.
PY - 2008/2
Y1 - 2008/2
N2 - To determine whether cones and Müller cells in the rod dominated retina cooperate to regenerate the 11-cis retinal chromophore via the retinoid cycle, two cell lines from the rod dominated retinas of Murine were used for this study: 661W, a mouse cell line derived from cones, and rMC-1, a rat Müller cell line. Retinoid cycle enzymes were analyzed by RT-PCR, and their catalytic activity was detected by incubation with retinoids and analyzed by HPLC. We found that 661W cells are capable of reducing all-trans retinal to all-trans retinol due to the presence of multiple dehydrogenases and to generate minor amounts of retinyl-ester. The rMC-1 cells take up all-trans retinol and oxidize it to all-trans retinal or esterify it to retinyl-ester, but are incapable of isomerizing all-trans retinoids to 11-cis retinoids. This could be a reflection of lack of necessary activities in Müller cells in vivo, which suggests that Müller cells do not contribute to retinoid cycling by regenerating 11-cis retinoids. Alternatively, this could be due to the potential that rMC-1, as a transformed cell line, has stopped expressing the proteins needed for the regeneration of 11-cis retinoids.
AB - To determine whether cones and Müller cells in the rod dominated retina cooperate to regenerate the 11-cis retinal chromophore via the retinoid cycle, two cell lines from the rod dominated retinas of Murine were used for this study: 661W, a mouse cell line derived from cones, and rMC-1, a rat Müller cell line. Retinoid cycle enzymes were analyzed by RT-PCR, and their catalytic activity was detected by incubation with retinoids and analyzed by HPLC. We found that 661W cells are capable of reducing all-trans retinal to all-trans retinol due to the presence of multiple dehydrogenases and to generate minor amounts of retinyl-ester. The rMC-1 cells take up all-trans retinol and oxidize it to all-trans retinal or esterify it to retinyl-ester, but are incapable of isomerizing all-trans retinoids to 11-cis retinoids. This could be a reflection of lack of necessary activities in Müller cells in vivo, which suggests that Müller cells do not contribute to retinoid cycling by regenerating 11-cis retinoids. Alternatively, this could be due to the potential that rMC-1, as a transformed cell line, has stopped expressing the proteins needed for the regeneration of 11-cis retinoids.
KW - cone photoreceptors
KW - retinal cell culture
KW - retinoid recycling
KW - retinoids
UR - http://www.scopus.com/inward/record.url?scp=39149142849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=39149142849&partnerID=8YFLogxK
U2 - 10.1016/j.exer.2007.11.006
DO - 10.1016/j.exer.2007.11.006
M3 - Article
C2 - 18163989
AN - SCOPUS:39149142849
SN - 0014-4835
VL - 86
SP - 344
EP - 354
JO - Experimental eye research
JF - Experimental eye research
IS - 2
ER -