Retinoid acid and taurine promote NeuroD1-induced differentiation of induced pluripotent stem cells into retinal ganglion cells

Li Huang, Mengfei Chen, Weizhong Zhang, Xuerong Sun, Bingqian Liu, Jian Ge

Research output: Contribution to journalArticlepeer-review

Abstract

Induced pluripotent stem cells (iPSCs) possess the capacity to differentiate into multiple cell types including retinal neurons. Despite substantial progress in the transcriptional regulation of iPSC differentiation process, the efficiency of generation of retinal neurons from iPSCs is still low. In this study, we investigated the role of transcription factor NeuroD1 in the differentiation of iPSCs into retinal neurons. We observed that retrovirus-mediated NeuroD1 overexpression in iPSCs increased the efficiency of neuronal differentiation. Immunostaining analysis showed that NeuroD1 overexpression increased the expression of retina ganglion cell markers including Islet-1, Math5, Brn3b, and Thy1.2. Retinoid acid (RA) and taurine further improved the differentiation efficiency of iPSCs overexpressing NeuroD1. However, RA and taurine did not promote differentiation in the absence of NeuroD1 overexpression. Together, our study provides new evidence in transcription factor-regulated stem cell differentiation in vitro.

Original languageEnglish (US)
Pages (from-to)67-76
Number of pages10
JournalMolecular and Cellular Biochemistry
Volume438
Issue number1-2
DOIs
StatePublished - Jan 1 2018

Keywords

  • NeuroD1
  • Pluripotent stem cells
  • Retinal ganglion cells
  • Retinoic acid
  • Taurine

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Retinoid acid and taurine promote NeuroD1-induced differentiation of induced pluripotent stem cells into retinal ganglion cells'. Together they form a unique fingerprint.

Cite this