Retinoic acid alters the proportions of high mannose to complex type oligosaccharides on fibronectin secreted by cultured chondrocytes

B. A. Bernard, Luigi M De Luca, J. R. Hassell, K. M. Yamada, K. Olden

Research output: Contribution to journalArticle

Abstract

Fibronectin synthesized by retinoid acid-treated chondrocytes exhibits a higher apparent subunit molecular weight in sodium dodecyl sulfate-polyacrylamide gels than fibronectin from untreated cultures. Analyses of peptide fragments show that retinoid acid treatment alters the apparent molecular weight of the collagen-binding fragment. Approximately one-sixth of the carbohydrate moieties on the collagen-binding fragment from control chondrocytes were sensitive to endo-β-N-acetylglucosaminidase H, whereas the collagen-binding fragment from treated chondrocytes was more resistant to endo-β-N-acetylglucosaminidase H. These findings indicate that chondrocyte fibronectin contains oligosaccharides of the high mannose or hybrid type in contrast to fibroblast fibronectin which only contains complex type carbohydrate chains and that retinoic acid treatment results in a higher proportion of complex type carbohydrate chains on chondrocyte fibronectin.

Original languageEnglish (US)
Pages (from-to)5310-5315
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number8
StatePublished - 1984
Externally publishedYes

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Mannose
Chondrocytes
Tretinoin
Oligosaccharides
Fibronectins
Acetylglucosaminidase
Collagen
Retinoids
Carbohydrates
Molecular Weight
Molecular weight
Peptide Fragments
Acids
Fibroblasts
Sodium Dodecyl Sulfate
endo-alpha-sialidase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Retinoic acid alters the proportions of high mannose to complex type oligosaccharides on fibronectin secreted by cultured chondrocytes. / Bernard, B. A.; De Luca, Luigi M; Hassell, J. R.; Yamada, K. M.; Olden, K.

In: Journal of Biological Chemistry, Vol. 259, No. 8, 1984, p. 5310-5315.

Research output: Contribution to journalArticle

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