Retinoblastoma gene mutations in primary human prostate cancer

Yoshinobu Kubota; Kiyoshi Fujinami; Hiroji Uemura; Yasushi Dobashi; Hiroshi Miyamoto; Yoshiko Iwasaki; Hitoshi Kitamura; Taro Shuin

doi:10.1002/pros.2990270604

Retinoblastoma gene mutations in primary human prostate cancer

Yoshinobu Kubota, Kiyoshi Fujinami, Hiroji Uemura, Yasushi Dobashi, Hiroshi Miyamoto, Yoshiko Iwasaki, Hitoshi Kitamura, Taro Shuin

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82 Scopus citations

Abstract

Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino‐acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis. © 1995 Wiley‐Liss, Inc.

Original language	English (US)
Pages (from-to)	314-320
Number of pages	7
Journal	The Prostate
Volume	27
Issue number	6
DOIs	https://doi.org/10.1002/pros.2990270604
State	Published - Dec 1995
Externally published	Yes

Keywords

RNA‐SSCP analysis
human prostate cancer
retinoblastoma gene mutation
tumor suppressor gene

ASJC Scopus subject areas

Oncology
Urology

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10.1002/pros.2990270604

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title = "Retinoblastoma gene mutations in primary human prostate cancer",

abstract = "Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino‐acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis. {\textcopyright} 1995 Wiley‐Liss, Inc.",

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T1 - Retinoblastoma gene mutations in primary human prostate cancer

AU - Kubota, Yoshinobu

AU - Fujinami, Kiyoshi

AU - Uemura, Hiroji

AU - Dobashi, Yasushi

AU - Miyamoto, Hiroshi

AU - Iwasaki, Yoshiko

AU - Kitamura, Hitoshi

AU - Shuin, Taro

PY - 1995/12

Y1 - 1995/12

N2 - Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino‐acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis. © 1995 Wiley‐Liss, Inc.

AB - Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino‐acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis. © 1995 Wiley‐Liss, Inc.

KW - RNA‐SSCP analysis

KW - human prostate cancer

KW - retinoblastoma gene mutation

KW - tumor suppressor gene

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Retinoblastoma gene mutations in primary human prostate cancer

Abstract

Keywords

ASJC Scopus subject areas

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