Retinoblastoma gene mutations in primary human prostate cancer

Yoshinobu Kubota, Kiyoshi Fujinami, Hiroji Uemura, Yasushi Dobashi, Hiroshi Miyamoto, Yoshiko Iwasaki, Hitoshi Kitamura, Taro Shuin

Research output: Contribution to journalArticlepeer-review

Abstract

Structural alterations in the entire coding regions (exons 1 to 27) of the retinoblastoma (RB) gene in primary human prostate cancers were investigated, using polymerase chain reaction and single strand conformational polymorphism analysis of RNA. Of 25 samples obtained from patients, four (16.4%) were found to have RB alterations. DNA sequencing of the PCR products revealed point mutations resulting in single amino‐acid substitutions of exons 6 and 19 in two cases, and base deletions of exons 8 and 17 in two cases. Two of four cases with RB mutations were moderately differentiated localized tumors and other two with RB mutations were poorly differentiated tumors with metastases. Our results suggest that RB gene mutation is involved in progression steps of prostate carcinogenesis. © 1995 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)314-320
Number of pages7
JournalThe Prostate
Volume27
Issue number6
DOIs
StatePublished - Dec 1995

Keywords

  • RNA‐SSCP analysis
  • human prostate cancer
  • retinoblastoma gene mutation
  • tumor suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Urology

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