Retinal ganglion cell death in experimental glaucoma and after axotomy occurs by apoptosis

H. A. Quigley, R. W. Nickells, L. A. Kerrigan, M. E. Pease, D. J. Thibault, D. J. Zack

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Purpose. To investigate whether retinal ganglion cell death in experimental glaucoma and after axotomy occurs by apoptosis. Methods. Chronic elevated eye pressure was produced in 20 monkey eyes, and the optic nerve was transected unilaterally in the orbit of 10 monkeys and 14 rabbits. Sixteen monkey and 14 rabbit eyes were studied as normal controls. Analytic methods included light and electron microscopy, histochemistry for DNA fragmentation (TUNEI, method), and DNA electrophoresis in agarose gels. Results. Dying ganglion cells in the experimental retinal exhibited morphologic features of apoptosis, including chromatin condensation and formation of apoptotic bodies. Cells with a positive reaction for DNA fragmentation were observed in eyes subjected to axotomy and experimental glaucoma but were only rarely encountered in control eyes. No evidence of internucleosomal fragmentation was detected electrophoretically, possibly because of the small proportion of cells that were dying at any given time. Conclusion. Some retinal ganglion cells injured by glaucoma and by axotomy die by apoptosis.

Original languageEnglish (US)
Pages (from-to)774-786
Number of pages13
JournalInvestigative Ophthalmology and Visual Science
Issue number5
StatePublished - Jan 1 1995



  • apoptosis
  • axotomy
  • glaucoma
  • optic nerve
  • retinal ganglion cells

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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