@article{df2b34f49a6e4e3ebf36358059530ce3,
title = "Results of a cytomegalovirus (CMV)-specific CD8 +/interferon- γ + cytokine flow cytometry assay correlate with clinical evidence of protective immunity in patients with AIDS with CMV retinitis",
abstract = "To evaluate the potential clinical utility of a cytomegalovirus (CMV)-specific CD8 +/interferon (IFN)-γ + cytokine flow cytometry (CFC) assay for patients with CMV retinitis (CMVR), stored peripheral blood mononuclear cell specimens were obtained from patients with active CMVR (i.e., having clinical evidence of absent CMV-protective immunity), as well as from highly active antiretroviral therapy-treated patients with CMVR who were able to discontinue anti-CMV therapy without subsequent progression of retinitis (i.e., having clinical evidence of restored CMV-protective immunity). Positive CD8 +/IFN-γ + T lymphocyte responses to CMV phosphoprotein 65 or immediate early peptide-pool stimulation were present in specimens from only 3 of 10 patients with active CMVR but were present in at least 1 specimen from all 20 patients with immunorestored CMVR, with a mean of 2.4 specimens/patient tested, spanning up to 6 months of observation (P = .0001). Among the patients with immunorestored CMVR, positive responses were present in all longitudinal specimens from 15 of the 20 patients. These data suggest that further testing of the CMV-specific CD8 +/IFN- γ + CFC assay, for clinical utility in predicting incident and progressive CMVR disease, is warranted.",
author = "Jacobson, {Mark A.} and Maecker, {Holden T.} and Orr, {Patricia L.} and Ron D'Amico and {Van Natta}, Mark and Li, {Xiao Dong} and Pollard, {Richard B.} and Bredt, {Barry M.}",
note = "Funding Information: Financial support: National Institutes of Health (NIH); University of California San Francisco (UCSF)–San Francisco General Hospital General Clinical Research Center (NIH grant MO1 RR00083); UCSF AIDS Clinical Trials Unit (NIH grant AI27663); AIDS Clinical Trials Group Immunology Support Laboratory (NIH grant UO1 AI38858); UCSF-Gladstone Institute of Virology and Immunology (GIVI) Center for AIDS Research (NIH grant P30 MH59037); UCSF/GIVI (NIH grants R01 AI47062 and R01 AI49538); cooperative agreements between the National Eye Institute and The Johns Hopkins University School of Medicine (NIH grant U10 EY08052), The Johns Hopkins University Bloomberg School of Public Health (NIH grant U10 EY08057), and the University of Wisconsin, Madison (NIH grant U10 EY08067); National Center for Research Resources (General Clinical Research Center grants 5MO1 RR00188 [Baylor College of Medicine], MO1 RR00052 [Johns Hopkins University School of Medicine], 5MO1 RR05096 [Louisiana State University], 5MO1 RR00865 [University of California, Los Angeles], 5MO1 RR05280 [University of Miami], 5M01 RR00046 [University of North Carolina], 5MO1 RR00043 [University of Southern California], and 5MO1 RR00047 [Weill Medical College of Cornell University]).",
year = "2004",
month = apr,
day = "15",
doi = "10.1086/382964",
language = "English (US)",
volume = "189",
pages = "1362--1373",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "8",
}