Restructuring the bacteriophage f1 genome

Expression of gene VIII in the intergenic space

P. B. Moses, J. D. Boeke, K. Horiuchi, N. D. Zinder

Research output: Contribution to journalArticle

Abstract

Using in vitro recombinant DNA techniques, we have constructed a derivative of bacteriophage f1 which contains an amber mutation in the major coat protein gene (gene VIII) plus an additional wild-type copy of this gene in the intergenic space near the origin of phage DNA replication. The gene is functional in this new location and is expressed at the same level relative to available transcriptional template DNA (RF) as the corresponding gene in wild-type f1. The implication of this finding in terms of current models of gene expression in the filamentous coliphages is discussed. Intertypic mixing of coat protein monomers derived from the two genes occurs when this phage is grown in an amber suppressor host. The effect of this DNA insertion on replication of the f1 genome is discussed. The concept of restructuring the f1 genome as a means of studying its regulation and expression is also discussed.

Original languageEnglish (US)
Pages (from-to)267-278
Number of pages12
JournalVirology
Volume104
Issue number2
StatePublished - 1980
Externally publishedYes

Fingerprint

Bacteriophages
Genome
Gene Expression
Genes
Capsid Proteins
Coliphages
Recombinant DNA
DNA
DNA Replication
Mutation

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Moses, P. B., Boeke, J. D., Horiuchi, K., & Zinder, N. D. (1980). Restructuring the bacteriophage f1 genome: Expression of gene VIII in the intergenic space. Virology, 104(2), 267-278.

Restructuring the bacteriophage f1 genome : Expression of gene VIII in the intergenic space. / Moses, P. B.; Boeke, J. D.; Horiuchi, K.; Zinder, N. D.

In: Virology, Vol. 104, No. 2, 1980, p. 267-278.

Research output: Contribution to journalArticle

Moses, PB, Boeke, JD, Horiuchi, K & Zinder, ND 1980, 'Restructuring the bacteriophage f1 genome: Expression of gene VIII in the intergenic space', Virology, vol. 104, no. 2, pp. 267-278.
Moses PB, Boeke JD, Horiuchi K, Zinder ND. Restructuring the bacteriophage f1 genome: Expression of gene VIII in the intergenic space. Virology. 1980;104(2):267-278.
Moses, P. B. ; Boeke, J. D. ; Horiuchi, K. ; Zinder, N. D. / Restructuring the bacteriophage f1 genome : Expression of gene VIII in the intergenic space. In: Virology. 1980 ; Vol. 104, No. 2. pp. 267-278.
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