TY - JOUR
T1 - Restriction of Toxoplasma gondii growth in human brain microvascular endothelial cells by activation of indoleamine 2,3-dioxygenase
AU - Däubener, W.
AU - Spors, B.
AU - Hucke, C.
AU - Adam, R.
AU - Stins, M.
AU - Kwang Sik Kim, Sik Kim
AU - Schroten, H.
PY - 2001
Y1 - 2001
N2 - One of the first steps in the development of cerebral toxoplasmosis is the penetration of the blood-brain barrier, which is comprised of microvascular endothelial cells. We examined the capacity of human brain microvascular endothelial cells (HBMEC) to interact with Toxoplasma gondii. We found that stimulation of HBMEC with gamma interferon (IFN-γ) resulted in the induction of toxoplasmostasis. The capacity of HBMEC to restrict Toxoplasma growth after IFN-γ stimulation was enhanced in the presence of tumor necrosis factor alpha (TNF-α). In addition, we found that IFN-γ induced a strong induction of indoleamine 2,3-dioxygenase (IDO) activity in HBMEC, and this enzyme activity was enhanced by costimulation with TNF-α. The addition of excess amounts of tryptophan to the HBMEC cultures resulted in a complete abrogation of the IFN-γ-TNF-α-mediated toxoplasmostasis. We therefore conclude that IDO induction contributed to the antiparasitic effector mechanism inducible in HBMEC by IFN-γ and TNF-α.
AB - One of the first steps in the development of cerebral toxoplasmosis is the penetration of the blood-brain barrier, which is comprised of microvascular endothelial cells. We examined the capacity of human brain microvascular endothelial cells (HBMEC) to interact with Toxoplasma gondii. We found that stimulation of HBMEC with gamma interferon (IFN-γ) resulted in the induction of toxoplasmostasis. The capacity of HBMEC to restrict Toxoplasma growth after IFN-γ stimulation was enhanced in the presence of tumor necrosis factor alpha (TNF-α). In addition, we found that IFN-γ induced a strong induction of indoleamine 2,3-dioxygenase (IDO) activity in HBMEC, and this enzyme activity was enhanced by costimulation with TNF-α. The addition of excess amounts of tryptophan to the HBMEC cultures resulted in a complete abrogation of the IFN-γ-TNF-α-mediated toxoplasmostasis. We therefore conclude that IDO induction contributed to the antiparasitic effector mechanism inducible in HBMEC by IFN-γ and TNF-α.
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U2 - 10.1128/IAI.69.10.6527-6531.2001
DO - 10.1128/IAI.69.10.6527-6531.2001
M3 - Article
C2 - 11553600
AN - SCOPUS:0034832976
SN - 0019-9567
VL - 69
SP - 6527
EP - 6531
JO - Infection and immunity
JF - Infection and immunity
IS - 10
ER -