Analysis of restriction fragment length polymorphism (RFLP), using a DNA probe directed against the insertion sequence IS6110, was applied to strains of Mycobacterium tuberculosis successively isolated from four patients. In order to determine the cause of recurrence in these patients, the RFLP patterns of the corresponding isolates were analyzed. The profiles obtained from the strains isolated from each of the patients were identical, thus suggesting that a relapse, rather than an exogenous reinfection with a new strain, was the cause of recurrence. The RFLP patterns of successive isolates remained unchanged during periods of time ranging from 5 months to 7 years, and were not modified after development of rifampin resistance. These results demonstrate the stability of the polymorphism detected by the IS6110 probe. Therefore, RFLP analysis is a powerful epidemiologic tool to distinguish relapse from exogenous reinfection.
|Translated title of the contribution||Restriction fragment length polymorphism of Mycobacterium tuberculosis strains isolated from patients with recurrence of tuberculosis|
|Number of pages||4|
|State||Published - Jul 3 1996|
- exogenous reinfection
ASJC Scopus subject areas
- Pathology and Forensic Medicine