Restricted immunoglobulin junctional diversity in neonatal B cells results from developmental selection rather than homology-based V(D)J joining

Akhilesh Pandey, Larry W. Tjoelker, Craig B. Thompson

Research output: Contribution to journalArticle

Abstract

The mechanism by which coding ends are joined during immunoglobulin (Ig) recombination is poorly understood. Recently, short sequence similarities (2-6 bp) observed at the ends of certain variable (V), diversity (D), and joining (J) gene segments of Ig have been correlated with limited junctional diversity observed in coding exons assembled from these elements. However, it is unclear whether these sequence homologies play any direct role in favoring coding joint formation by influencing the V(D)J recombination process. In this report, we demonstrate that coding sequence similarities do not influence the position of coding joints during V(D)J recombination in vivo. Instead, during embryonic development, B cells with certain joining products undergo progressive selection. Developmental selection is completed before exposure to external antigens and appears to be determined by the amino acid sequence encoded by the coding joint. We conclude that the nucleotide sequences of the coding regions do not play a major role in directing V(D)J recombination. Instead, we propose that limited Ig junctional diversity results from prenatal developmental selection of B cells based on the protein sequence of their surface Ig antigen-binding site. Sequence identities at the ends of coding segments may have evolved because they increase the likelihood that a selectable antigen-binding site is created during a random recombination process.

Original languageEnglish (US)
Pages (from-to)329-337
Number of pages9
JournalJournal of Experimental Medicine
Volume177
Issue number2
StatePublished - Feb 1 1993
Externally publishedYes

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V(D)J Recombination
Immunoglobulins
B-Lymphocytes
Joints
Genetic Recombination
Binding Sites
Antigens
B-Cell Antigen Receptors
Immunoglobulin Genes
Surface Antigens
Sequence Homology
Embryonic Development
Amino Acid Sequence
Exons
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Restricted immunoglobulin junctional diversity in neonatal B cells results from developmental selection rather than homology-based V(D)J joining. / Pandey, Akhilesh; Tjoelker, Larry W.; Thompson, Craig B.

In: Journal of Experimental Medicine, Vol. 177, No. 2, 01.02.1993, p. 329-337.

Research output: Contribution to journalArticle

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