Restoration of HIV-specific cell-mediated immune responses by interleukin-12 in vitro

Mario Clerici, Daniel R. Lucey, Jay A. Berzofsky, Ligia A. Pinto, Thomas A. Wynn, Stephen P. Blatt, Matthew J. Dolan, Craig W. Hendrix, Stanley F. Wolf, Gene M. Shearer

Research output: Contribution to journalArticle

Abstract

Peripheral blood mononuclear cells (PBMCs) from many asymptomatic individuals infected with human immunodeficiency virus-type 1 (HIV) are unresponsive as measured by in vitro T cell proliferation and interleukin-2 (IL-2) production to influenza virus and synthetic peptides of HIV envelope (Env). Strong influenza virus- and Env-stimulated IL-2 responses and T cell proliferation were restored when cultures were stimulated in the presence of IL-12. Interferon-γ production by PBMCs from HIV seropositive (HIV +) patients was also restored with IL-12. Furthermore, in vitro antigen-specific production of IL-2 and proliferation of PBMCs from HIV - donors were suppressed by antibody to IL-12, but were not enhanced by addition of exogenous IL-12. Thus, IL12 may be limiting in PBMCs from HIV+ but not HIV- individuals. These findings demonstrate that IL-12 can restore HIV-specific cell-mediated immunity in vitro in HIV-infected individuals and suggest a potential use of IL-12 in augmenting the diminished immunologic functions associated with HIV infection.

Original languageEnglish (US)
Pages (from-to)1721-1724
Number of pages4
JournalScience
Volume262
Issue number5140
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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    Clerici, M., Lucey, D. R., Berzofsky, J. A., Pinto, L. A., Wynn, T. A., Blatt, S. P., Dolan, M. J., Hendrix, C. W., Wolf, S. F., & Shearer, G. M. (1993). Restoration of HIV-specific cell-mediated immune responses by interleukin-12 in vitro. Science, 262(5140), 1721-1724. https://doi.org/10.1126/science.7903123