Responses of brain and non-brain endothelial cells to meningitis-causing Escherichia coli K1

Maneesh Paul-Satyaseela, Yi Xie, Francescopaolo Di Cello, Kwang Sik Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Bacterial interaction with specific host tissue may contribute to its propensity to cause an infection in a particular site. In this study, we examined whether meningitis-causing Escherichia coli K1 interaction with human brain microvascular endothelial cells, which constitute the blood-brain barrier, differed from its interaction with non-brain endothelial cells derived from skin and umbilical cord. We showed that E. coli K1 association was significantly greater with human brain microvascular endothelial cells than with non-brain endothelial cells. In addition, human brain microvascular endothelial cells maintained their morphology and intercellular junctional resistance in response to E. coli K1. In contrast, non-brain endothelial cells exhibited decreased transendothelial electrical resistance and detachment from the matrix upon exposure to E. coli K1. These different responses of brain and non-brain endothelial cells to E. coli K1 may form the basis of E. coli K1's propensity to cause meningitis.

Original languageEnglish (US)
Pages (from-to)81-85
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume342
Issue number1
DOIs
StatePublished - Mar 31 2006

Keywords

  • Endothelial cells
  • Escherichia coli
  • HBMEC
  • HDMEC
  • HMEC
  • HUVEC
  • Meningitis
  • TEER
  • Tissue tropism

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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