Response of Pig Retinal Pigment Epithelium to Laser Photocoagulation in Organ Culture

Lucian V. Del Priore, Bert M. Glaser, Harry A Quigley, W. Richard Green

Research output: Contribution to journalArticle

Abstract

Laser photocoagulation was applied in vitro to organ culture exoplants of porcine retinal pigment epithelium (RPE) attached to Bruch's membrane. Four-millimeter-round buttons of eye wall containing RPE, choroid, and sclera were treated with 25 spots from the argon blue-green laser using 300 mW of power, a 500-μm spot size, and 0.1-s duration. Laser photocoagulation disrupts individual RPE cells acutely and lifts damaged RPE cells from Bruch's membrane. Treated areas become covered with irregular mounds of RPE cells within seven days. The acute damage and subsequent repair of the RPE in organ culture mimic the response of the RPE following laser photocoagulation in vivo. Thus, the morphologic response of the RPE to laser photocoagulation is an intrinsic property of this tissue that does not depend on the presence of the overlying retina.

Original languageEnglish (US)
Pages (from-to)119-122
Number of pages4
JournalArchives of Ophthalmology
Volume107
Issue number1
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Retinal Pigment Epithelium
Light Coagulation
Organ Culture Techniques
Lasers
Swine
Bruch Membrane
Sclera
Choroid
Argon
Retina
Cell Membrane

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Response of Pig Retinal Pigment Epithelium to Laser Photocoagulation in Organ Culture. / Del Priore, Lucian V.; Glaser, Bert M.; Quigley, Harry A; Green, W. Richard.

In: Archives of Ophthalmology, Vol. 107, No. 1, 1989, p. 119-122.

Research output: Contribution to journalArticle

Del Priore, Lucian V. ; Glaser, Bert M. ; Quigley, Harry A ; Green, W. Richard. / Response of Pig Retinal Pigment Epithelium to Laser Photocoagulation in Organ Culture. In: Archives of Ophthalmology. 1989 ; Vol. 107, No. 1. pp. 119-122.
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