Respiratory syncytial virus stimulation of vascular endothelial cell growth factor/vascular permeability factor

C. G. Lee, H. J. Yoon, Z. Zhu, H. Link, Z. Wang, Jr Gwaltney J.M., M. Landry, J. A. Elias

Research output: Contribution to journalArticle

Abstract

We hypothesized that respiratory syncytial virus (RSV)-induced pathologies could be mediated, in part, by vascular active cytokines elaborated during virus infection. To address this hypothesis, we determined whether RSV stimulated vascular endothelial cell growth factor (VEGF)/vascular permeability factor (VPF) elaboration in vitro. Supernatants from un-stimulated A549 cells and normal human bronchial epithelial cells contained modest levels of VEGF. In contrast, supernatants from RSV-infected cells contained elevated levels of VEGF/VPF. This stimulation was seen after as little as 2 h, was still prominent after 48 h, and, by immunoblot, was specific for the 165- and 121-amino acid isoforms of VEGF/VPF. It was not associated with significant cell cytotoxicity or alterations in VEGF messenger RNA. It did, however, require new protein biosynthesis. In accordance with these findings, the 165- and 121-amino acid isoforms of VFGF/VPF were also found in the nasal washings from patients with RSV infections. These studies demonstrate that RSV is a potent stimulator of VEGF/VPF elaboration and that, in vitro, this stimulation is mediated via a noncytotoxic translational and/or post-translational biosynthetic mechanism. VEGF/VPF may play an important role in the pathogenesis of RSV-induced disorders.

Original languageEnglish (US)
Pages (from-to)662-669
Number of pages8
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume23
Issue number5
StatePublished - 2000
Externally publishedYes

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Respiratory Syncytial Viruses
Endothelial cells
Cell growth
Viruses
Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
Protein Isoforms
Amino Acids
Biosynthesis
Pathology
Cytotoxicity
Washing
Nasal Lavage Fluid
Respiratory Syncytial Virus Infections
Cytokines
Messenger RNA
Protein Biosynthesis
Virus Diseases
Blood Vessels

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Lee, C. G., Yoon, H. J., Zhu, Z., Link, H., Wang, Z., Gwaltney J.M., J., ... Elias, J. A. (2000). Respiratory syncytial virus stimulation of vascular endothelial cell growth factor/vascular permeability factor. American Journal of Respiratory Cell and Molecular Biology, 23(5), 662-669.

Respiratory syncytial virus stimulation of vascular endothelial cell growth factor/vascular permeability factor. / Lee, C. G.; Yoon, H. J.; Zhu, Z.; Link, H.; Wang, Z.; Gwaltney J.M., Jr; Landry, M.; Elias, J. A.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 23, No. 5, 2000, p. 662-669.

Research output: Contribution to journalArticle

Lee, CG, Yoon, HJ, Zhu, Z, Link, H, Wang, Z, Gwaltney J.M., J, Landry, M & Elias, JA 2000, 'Respiratory syncytial virus stimulation of vascular endothelial cell growth factor/vascular permeability factor', American Journal of Respiratory Cell and Molecular Biology, vol. 23, no. 5, pp. 662-669.
Lee, C. G. ; Yoon, H. J. ; Zhu, Z. ; Link, H. ; Wang, Z. ; Gwaltney J.M., Jr ; Landry, M. ; Elias, J. A. / Respiratory syncytial virus stimulation of vascular endothelial cell growth factor/vascular permeability factor. In: American Journal of Respiratory Cell and Molecular Biology. 2000 ; Vol. 23, No. 5. pp. 662-669.
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