Respiratory syncytial virus serology by a simplified enzyme-linked immunosorbent assay

M. C. Steinhoff, C. B. Hall, K. C. Schnabel

Research output: Contribution to journalArticle

Abstract

A simplified enzyme-linked immunosorbent assay (ELISA) which utilized commercially available reagents was developed for respiratory syncytial virus (RSV)-specific immunoglobulin G. An analysis of the inherent variation of the assay allowed the setting of strict criteria for determining a significant change in RSV antibodies. The ELISA was more sensitive than the standard complement fixation or microneutralization tests in a carefully studied group of 32 RSV-infected adults. The ELISA correlated closely with complement fixation serological testing in 25 patients. The use of purified antigens might allow the development of a more sensitive ELISA.

Original languageEnglish (US)
Pages (from-to)447-450
Number of pages4
JournalJournal of clinical microbiology
Volume12
Issue number3
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Microbiology (medical)

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