Resolution of ambiguous low-level positive quantitative polymerase chain reaction results in TEL-AML1 positive ALL using a post-PCR fluorescent oligoligation method

I. Ming Chen, Artemis Chakerian, Meenakshi Devidas, Michael J. Borowitz, Stephen P. Hunger, Cheryl L. Willman, David S. Viswanatha

Research output: Contribution to journalArticle

Abstract

The interpretation of low-level or non-reproducible amplification results in clinical quantitative polymerase chain reaction (Q-PCR) assays can be difficult to definitively resolve. Concerning minimal residual disease detection in leukaemia, indeterminate low-level results might create prognostic or therapeutic dilemmas. We evaluated low-level, ambiguous Q-PCR results in a study of paired diagnostic and end-induction (day 29) TEL-AML1 positive acute lymphoblastic leukaemia samples utilising a novel fluorescent primer ligation detection assay. The data presented here indicate that a significant number of low-level apparent Q-PCR positive results may be spurious or non-specific in nature, requiring additional technical manoeuvres for confirmation of true positive cases.

Original languageEnglish (US)
Pages (from-to)358-361
Number of pages4
JournalBritish journal of haematology
Volume135
Issue number3
DOIs
StatePublished - Nov 2006

Keywords

  • Fluorescent ligation detection
  • Minimal residual disease
  • Quantitative polymerase chain reaction
  • TEL-AML1

ASJC Scopus subject areas

  • Hematology

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