Rescue of the Drosophila phototransduction mutation trp by germline transformation

Craig Montell, Kevin Jones, Ernst Hafen, Gerald Rubin

Research output: Contribution to journalArticle

Abstract

Phototransduction is the process by which light-stimulated photoreceptor cells of the visual system send electrical signals to the nervous system. Many of the steps that follow the initial event in phototransduction, absorption of light by rhodopsin, are ill-defined. The fruitfly, Drosophila melanogaster, provides a means to dissect phototransduction genetically. Mutations such as transient receptor potential (trp) affect intermediate steps in phototransduction. In order to facilitate molecular studies of phototransduction, the trp gene was isolated and its identity was confirmed by complementing the mutant trpCM allele of the trp gene by P-element mediated germline transformation of a 7.1-kilobase DNA fragment. Expression of the trp gene begins late in pupal development and appears to be limited to the eyes and ocelli.

Original languageEnglish (US)
Pages (from-to)1040-1043
Number of pages4
JournalScience
Volume230
Issue number4729
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

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    Montell, C., Jones, K., Hafen, E., & Rubin, G. (1985). Rescue of the Drosophila phototransduction mutation trp by germline transformation. Science, 230(4729), 1040-1043.