Requirements for self-splicing of a group I intron from Physarum polycephalum

Gail Auger Rocheleau, Sarah A. Woodson

Research output: Contribution to journalArticle

Abstract

The third intron from Physarum polycephalum (Pp LSU 3) is one of the closest known relatives to the wellstudied Tetrahymena group I intron. Both introns are located at the same position in the 26S rRNA gene, and with the exception of an open reading frame in Pp LSU 3, are highly homologous. While Pp LSU 3 has been shown to self splice, little is known about its activity in vitro. We have examined the requirements for self splicing in greater detail. Despite its similarity to the Tetrahymena intron, Pp LSU 3 is 1500-fold less reactive, demonstrates a preference for high salt, and exhibits a low Km for GTP. Removal of the open reading frame results in a modest increase of activity. This system provides an opportunity to understand how sequence variations in two related introns alter the efficiency of autoexcision, and how this relates to adaptation of group I introns to their particular sequence context.

Original languageEnglish (US)
Pages (from-to)4315-4320
Number of pages6
JournalNucleic acids research
Volume22
Issue number20
DOIs
StatePublished - Oct 11 1994

ASJC Scopus subject areas

  • Genetics

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