Requirement of posttranslational modifications for the generation of biologic activity of glycosylation-inhibiting factor

Yun Cai Liu, Tatsumi Nakano, Christian Elly, Kimishige Ishizaka

Research output: Contribution to journalArticlepeer-review

Abstract

Secretion of bioactive glycosylation-inhibiting factor (GIF) appears to be restricted to suppressor T (T(s)) cells, although various human and murine cell line cells secrete the 13-kDa peptide that reacts with anti-GIF. Nucleotide sequences of GIF cDNA from the T(s) and non-T(s) cells are identical, indicating that bioactive GIF and inactive GIF have an identical amino acid sequence. A stable transfectant of human GIF (hGIF) cDNA in BMT10 cells secretes inactive GIF peptide, whereas transfection of a chimeric cDNA encoding a fusion protein consisting of the N-terminal region of procalcitonin precursor and hGIF into the same cells results in secretion of bioactive GIF. Evidence was obtained that the fusion protein goes into the endoplasmic reticulum and is cleaved for the secretion of mature GIF peptide, whereas the inactive 13-kDa peptide synthesized by the former transfectant does not go through the endoplasmic reticulum. However, a stable transfectant of hGIF cDNA in mouse T(s) hybridoma contains inactive GIF in the cytosol and secretes bioactive hGIF without participation of the endoplasmic reticulum- Golgi system. Heterogeneity of the 13-kDa hGIF from the transfectant was detected in two-dimensional electrophoresis. The results suggested that T(s) cells have a machinery that converts a portion of inactive cytosolic GIF peptide to bioactive GIF during secretion.

Original languageEnglish (US)
Pages (from-to)11227-11231
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number23
DOIs
StatePublished - Nov 8 1994
Externally publishedYes

Keywords

  • endoplasmic reticulum
  • macrophage-migration inhibitory factor
  • suppressor T cells

ASJC Scopus subject areas

  • General

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