Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression

Research output: Contribution to journalArticle

Abstract

Cerebellar long-term depression (LTD) is a model of synaptic memory that requires protein kinase C (PKC) activation and is expressed as a reduction in the number of postsynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. LTD was absent in cultured cerebellar Purkinje cells from mutant mice lacking the AMPA receptor GluR2 subunit and could be rescued by transient transfection with the wild-type GluR2 subunit. Transfection with a point mutant that eliminated PKC phosphorylation of Ser880 in the carboxy-terminal PDZ ligand of GluR2 failed to restore LTD. In contrast, transfection with a point mutant that mimicked phosphorylation at Ser880 occluded subsequent LTD. Thus, PKC phosphorylation of GluR2 Ser880 is a critical event in the induction of cerebellar LTD.

Original languageEnglish (US)
Pages (from-to)1751-1755
Number of pages5
JournalScience
Volume300
Issue number5626
DOIs
StatePublished - Jun 13 2003

Fingerprint

AMPA Receptors
Phosphorylation
Depression
Protein Kinase C
Transfection
Purkinje Cells
Mutant Proteins
Ligands

ASJC Scopus subject areas

  • General

Cite this

Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. / Chung, Hee Jung; Steinberg, Jordan; Huganir, Richard L; Linden, David J.

In: Science, Vol. 300, No. 5626, 13.06.2003, p. 1751-1755.

Research output: Contribution to journalArticle

@article{0632d52c60bb4b86a21110a83f543a2e,
title = "Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression",
abstract = "Cerebellar long-term depression (LTD) is a model of synaptic memory that requires protein kinase C (PKC) activation and is expressed as a reduction in the number of postsynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. LTD was absent in cultured cerebellar Purkinje cells from mutant mice lacking the AMPA receptor GluR2 subunit and could be rescued by transient transfection with the wild-type GluR2 subunit. Transfection with a point mutant that eliminated PKC phosphorylation of Ser880 in the carboxy-terminal PDZ ligand of GluR2 failed to restore LTD. In contrast, transfection with a point mutant that mimicked phosphorylation at Ser880 occluded subsequent LTD. Thus, PKC phosphorylation of GluR2 Ser880 is a critical event in the induction of cerebellar LTD.",
author = "Chung, {Hee Jung} and Jordan Steinberg and Huganir, {Richard L} and Linden, {David J}",
year = "2003",
month = "6",
day = "13",
doi = "10.1126/science.1082915",
language = "English (US)",
volume = "300",
pages = "1751--1755",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5626",

}

TY - JOUR

T1 - Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression

AU - Chung, Hee Jung

AU - Steinberg, Jordan

AU - Huganir, Richard L

AU - Linden, David J

PY - 2003/6/13

Y1 - 2003/6/13

N2 - Cerebellar long-term depression (LTD) is a model of synaptic memory that requires protein kinase C (PKC) activation and is expressed as a reduction in the number of postsynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. LTD was absent in cultured cerebellar Purkinje cells from mutant mice lacking the AMPA receptor GluR2 subunit and could be rescued by transient transfection with the wild-type GluR2 subunit. Transfection with a point mutant that eliminated PKC phosphorylation of Ser880 in the carboxy-terminal PDZ ligand of GluR2 failed to restore LTD. In contrast, transfection with a point mutant that mimicked phosphorylation at Ser880 occluded subsequent LTD. Thus, PKC phosphorylation of GluR2 Ser880 is a critical event in the induction of cerebellar LTD.

AB - Cerebellar long-term depression (LTD) is a model of synaptic memory that requires protein kinase C (PKC) activation and is expressed as a reduction in the number of postsynaptic α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. LTD was absent in cultured cerebellar Purkinje cells from mutant mice lacking the AMPA receptor GluR2 subunit and could be rescued by transient transfection with the wild-type GluR2 subunit. Transfection with a point mutant that eliminated PKC phosphorylation of Ser880 in the carboxy-terminal PDZ ligand of GluR2 failed to restore LTD. In contrast, transfection with a point mutant that mimicked phosphorylation at Ser880 occluded subsequent LTD. Thus, PKC phosphorylation of GluR2 Ser880 is a critical event in the induction of cerebellar LTD.

UR - http://www.scopus.com/inward/record.url?scp=0038518145&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038518145&partnerID=8YFLogxK

U2 - 10.1126/science.1082915

DO - 10.1126/science.1082915

M3 - Article

C2 - 12805550

AN - SCOPUS:0038518145

VL - 300

SP - 1751

EP - 1755

JO - Science

JF - Science

SN - 0036-8075

IS - 5626

ER -