Requirement for the second coding exon of Tat in the optimal replication of macrophage-tropic HIV-1

Christine Neuveut, Robert M. Scoggins, David Camerini, Richard B. Markham, Kuan Teh Jeang

Research output: Contribution to journalArticlepeer-review

Abstract

HIV-1 Tat is essential for virus replication and is a potent transactivator of viral gene expression. Evidence suggests that Tat also influences virus infectivity and cytopathicity. Here, we find that the second coding exon of Tat contributes a novel function for the replication/infectivity of macrophage-tropic HIV-1. We show that macrophage-tropic HIV-1 which expresses the full-length two-exon form of Tat replicates better in monocyte-derived macrophages (MDM) than an otherwise isogenic virus which expresses only the one-exon form of Tat. Similarly, two-exon Tat expressing HIV-1 also replicates better than one-exon Tat expressing HIV-1 in two different models of human cells/tissue reconstituted SCID mice.

Original languageEnglish (US)
Pages (from-to)651-660
Number of pages10
JournalJournal of biomedical science
Volume10
Issue number6
DOIs
StatePublished - 2003

Keywords

  • AIDS pathogenesis
  • HIV
  • Macrophage tropism
  • SCID mouse
  • Tat

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

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