Requirement for p53 and p21 to sustain G2 arrest after DNA damage

F. Bunz, A. Dutriaux, C. Lengauer, T. Waldman, S. Zhou, J. P. Brown, J. M. Sedivy, K. W. Kinzler, B. Vogelstein

Research output: Contribution to journalArticle

Abstract

After DNA damage, many cells appear to enter a sustained arrest in the G2 phase of the cell cycle. It is shown here that this arrest could be sustained only when p53 was present in the cell and capable of transcriptionally activating the cyclin-dependent kinase inhibitor p21. After disruption of either the p53 or the p21 gene, γ radiated cells progressed into mitosis and exhibited a G2 DNA content only because of a failure of cytokinesis. Thus, p53 and p21 appear to be essential for maintaining the G2 checkpoint in human cells.

Original languageEnglish (US)
Pages (from-to)1497-1501
Number of pages5
JournalScience
Volume282
Issue number5393
DOIs
StatePublished - Nov 20 1998

ASJC Scopus subject areas

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