Requirement for generation of H2O2 for platelet-derived growth factor signal transduction

Maitrayee Sundaresan; Zu Xi Yu; Victor J. Ferrans; Kaikobad Irani; Toren Finkel

Requirement for generation of H2O2 for platelet-derived growth factor signal transduction

Maitrayee Sundaresan, Zu Xi Yu, Victor J. Ferrans, Kaikobad Irani, Toren Finkel

Research output: Contribution to journal › Article › peer-review

Abstract

Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H₂O₂). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H₂O₂ concentration was blocked. These results suggest that H₂O₂ may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.

Original language	English (US)
Pages (from-to)	296-299
Number of pages	4
Journal	Science
Volume	270
Issue number	5234
State	Published - 1995
Externally published	Yes

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title = "Requirement for generation of H2O2 for platelet-derived growth factor signal transduction",

abstract = "Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H2O2). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H2O2 concentration was blocked. These results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.",

author = "Maitrayee Sundaresan and Yu, {Zu Xi} and Ferrans, {Victor J.} and Kaikobad Irani and Toren Finkel",

year = "1995",

language = "English (US)",

volume = "270",

pages = "296--299",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5234",

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T1 - Requirement for generation of H2O2 for platelet-derived growth factor signal transduction

AU - Sundaresan, Maitrayee

AU - Yu, Zu Xi

AU - Ferrans, Victor J.

AU - Irani, Kaikobad

AU - Finkel, Toren

PY - 1995

Y1 - 1995

N2 - Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H2O2). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H2O2 concentration was blocked. These results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.

AB - Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H2O2). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H2O2 concentration was blocked. These results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.

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M3 - Article

C2 - 7569979

AN - SCOPUS:0028973482

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VL - 270

SP - 296

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JO - Science

JF - Science

IS - 5234

ER -

Requirement for generation of H2O2 for platelet-derived growth factor signal transduction

Abstract

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