Requirement for CRIF1 in RNA interference and Dicer-2 stability

Su Jun Lim, Anthony Scott, Xiao Peng Xiong, Shabnam Vahidpour, John Karijolich, Dongdong Guo, Shanshan Pei, Yi Tao Yu, Rui Zhou, Willis X. Li

Research output: Contribution to journalArticle

Abstract

RNA interference (RNAi) is a eukaryotic gene-silencing system. Although the biochemistry of RNAi is relatively well defined, how this pathway is regulated remains incompletely understood. To identify genes involved in regulating the RNAi pathway, we screened for genetic mutations in Drosophila that alter the efficiency of RNAi. We identified the Drosophila homolog of the mammalian CR6-interacting factor 1 (CRIF1), also known as growth arrest and DNA-damage-inducible 45-gamma interacting protein (Gadd45GIP1), as a potential new regulator of the RNAi pathway. Loss-of-function mutants of Drosophila CRIF1 (dCRIF) are deficient in RNAi-mediated target gene knock-down, in the biogenesis of small interfering RNA (siRNA) molecules, and in antiviral immunity. Moreover, we show that dCRIF may function by interacting with, and stabilizing, the RNase III enzyme Dicer-2. Our results suggest that dCRIF may play an important role in regulating the RNAi pathway.

Original languageEnglish (US)
Pages (from-to)1171-1179
Number of pages9
JournalRNA Biology
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2014

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Keywords

  • Dcr-2
  • Drosophila
  • Gadd45GIP
  • RNAi

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Lim, S. J., Scott, A., Xiong, X. P., Vahidpour, S., Karijolich, J., Guo, D., Pei, S., Yu, Y. T., Zhou, R., & Li, W. X. (2014). Requirement for CRIF1 in RNA interference and Dicer-2 stability. RNA Biology, 11(9), 1171-1179. https://doi.org/10.4161/rna.34381