Reprogramming somatic cells into pluripotent stem cells using miRNAs

Research output: Contribution to journalArticle

Abstract

Reversal of terminally differentiated somatic cells to ground-state pluripotency has rejuvenated our hopes of generating patient-specific stem cells for therapeutic use in regenerative medicine and drug screening. Originally generated using defined exogenous protein-coding DNA, several methods have been described in reprogramming somatic cells into iPSC. Majority of published methods seek to improve or refine the techniques of reprogramming. This chapter describes reprogramming to pluripotency using miRNAs.

Original languageEnglish (US)
Pages (from-to)273-281
Number of pages9
JournalMethods in Molecular Biology
Volume1150
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Pluripotent Stem Cells
MicroRNAs
Preclinical Drug Evaluations
Regenerative Medicine
Therapeutic Uses
Stem Cells
DNA
Proteins
Cellular Reprogramming

Keywords

  • Fibroblast
  • Induced pluripotent stem cells (iPSCs)
  • Lentivirus
  • MicroRNAs (miRNAs)
  • Reprogramming

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Reprogramming somatic cells into pluripotent stem cells using miRNAs. / Anokye-Danso, Frederick.

In: Methods in Molecular Biology, Vol. 1150, 2014, p. 273-281.

Research output: Contribution to journalArticle

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