Reprogramming of EBV-immortalized B-lymphocyte cell lines into induced pluripotent Stem cells

Su Mi Choi, Hua Liu, Pooja Chaudhari, Yonghak Kim, Linzhao Cheng, Jian Feng, Saul Sharkis, Zhaohui Ye, Yoon Young Jang

Research output: Contribution to journalArticlepeer-review

Abstract

EBV-immortalized B lymphocyte cell lines have been widely banked for studying a variety of diseases, including rare genetic disorders. These cell lines represent an important resource for disease modeling with the induced pluripotent stem cell (iPSC) technology. Here we report the generation of iPSCs from EBV-immortalized B-cell lines derived from multiple inherited disease patients via a nonviral method. The reprogramming method for the EBV cell lines involves a distinct protocol compared with that of patient fibroblasts. The B-cell line-derived iPSCs expressed pluripotency markers, retained the inherited mutation and the parental V(D)J rearrangement profile, and differentiated into all 3 germ layer cell types. There was no integration of the reprogramming-related transgenes or the EBV-associated genes in these iPSCs. The ability to reprogram the widely banked patient B-cell lines will offer an unprecedented opportunity to generate human disease models and provide novel drug therapies.

Original languageEnglish (US)
Pages (from-to)1801-1805
Number of pages5
JournalBlood
Volume118
Issue number7
DOIs
StatePublished - Aug 18 2011

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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