Reproducibility of tract-specific magnetization transfer and diffusion tensor imaging in the cervical spinal cord at 3 tesla

Seth A. Smith, Craig Jones, Aliya Gifford, Visar Belegu, BettyAnn Chodkowski, Jonathan A D Farrell, Bennett A. Landman, Daniel S. Reich, Peter Calabresi, John W. McDonald, Peter C Van Zijl

Research output: Contribution to journalArticle


Damage to specific white matter tracts within the spinal cord can often result in the particular neurological syndromes that characterize myelopathies such as traumatic spinal cord injury. Noninvasive visualization of these tracts with imaging techniques that are sensitive to microstructural integrity is an important clinical goal. Diffusion tensor imaging (DTI)- and magnetization transfer (MT)-derived quantities have shown promise in assessing tissue health in the central nervous system. In this paper, we demonstrate that DTI of the cervical spinal cord can reliably discriminate sensory (dorsal) and motor (lateral) columns. From data derived from nine healthy volunteers, two raters quantified column-specific parallel (λ∥) and perpendicular (λ⊥) diffusivity, fractional anisotropy (FA), mean diffusivity (MD), and MT-weighted signal intensity relative to cerebrospinal fluid (MTCSF) over two time-points separated by more than 1 week. Cross-sectional means and standard deviations of these measures in the lateral and dorsal columns were as follows: λ∥: 2.13±0.14 and 2.14±0.11μm 2/ms; λ⊥: 0.67±0.16 and 0.61±0.09μm 2/ms; MD: 1.15±0.15 and 1.12±0.08μm2/ms; FA: 0.68±0.06 and 0.68±0.05; MTCSF: 0.52±0.05 and 0.50±0.05.We examined the variability and interrater and test-retest reliability for each metric. These column-specific MR measurements are expected to enhance understanding of the intimate structure-function relationship in the cervical spinal cord and may be useful for the assessment of disease progression.

Original languageEnglish (US)
Pages (from-to)207-217
Number of pages11
JournalNMR in Biomedicine
Issue number2
StatePublished - Feb 2010



  • Diffusion tensor imaging
  • Magnetic resonance imaging
  • Magnetization transfer
  • Spinal cord
  • Tractography

ASJC Scopus subject areas

  • Spectroscopy
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

Smith, S. A., Jones, C., Gifford, A., Belegu, V., Chodkowski, B., Farrell, J. A. D., Landman, B. A., Reich, D. S., Calabresi, P., McDonald, J. W., & Van Zijl, P. C. (2010). Reproducibility of tract-specific magnetization transfer and diffusion tensor imaging in the cervical spinal cord at 3 tesla. NMR in Biomedicine, 23(2), 207-217.