Reproducibility of quantitative tractography methods applied to cerebral white matter

Setsu Wakana, Arvind Caprihan, Martina M. Panzenboeck, James H. Fallon, Michele Perry, Randy L. Gollub, Kegang Hua, Jiangyang Zhang, Hangyi Jiang, Prachi Dubey, Ari Blitz, Peter van Zijl, Susumu Mori

Research output: Contribution to journalArticlepeer-review

1048 Scopus citations


Tractography based on diffusion tensor imaging (DTI) allows visualization of white matter tracts. In this study, protocols to reconstruct eleven major white matter tracts are described. The protocols were refined by several iterations of intra- and inter-rater measurements and identification of sources of variability. Reproducibility of the established protocols was then tested by raters who did not have previous experience in tractography. The protocols were applied to a DTI database of adult normal subjects to study size, fractional anisotropy (FA), and T2 of individual white matter tracts. Distinctive features in FA and T2 were found for the corticospinal tract and callosal fibers. Hemispheric asymmetry was observed for the size of white matter tracts projecting to the temporal lobe. This protocol provides guidelines for reproducible DTI-based tract-specific quantification.

Original languageEnglish (US)
Pages (from-to)630-644
Number of pages15
Issue number3
StatePublished - Jul 1 2007


  • Diffusion tensor imaging
  • Reproducibility
  • Tractography
  • White matter

ASJC Scopus subject areas

  • Neurology
  • Cognitive Neuroscience


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