TY - JOUR
T1 - Reproducibility of extracellular vesicle research
AU - Nieuwland, Rienk
AU - Siljander, Pia R.M.
AU - Falcón-Pérez, Juan M.
AU - Witwer, Kenneth W.
N1 - Funding Information:
RN and PRMS are supported by the European Association of National Metrology Institutes , Grant/Award Number: 18HLT01 METVES II . PRMS is supported by The Academy of Finland (grant #330486, #337641 ), Magnus Ehrnrooth Foundation , Finnish Red Cross Blood Service Foundation and Business Finland ( EVE consortium ).
Publisher Copyright:
© 2022
PY - 2022/8
Y1 - 2022/8
N2 - Cells release membrane-delimited particles into the environment. These particles are called “extracellular vesicles” (EVs), and EVs are present in fluids contacting cells, including body fluids and conditioned culture media. Because EVs change and contribute to health and disease, EVs have become a hot topic. From the thousands of papers now published on EVs annually, one easily gets the impression that EVs provide biomarkers for all diseases, and that EVs are carriers of all relevant biomolecules and are omnipotent therapeutics. At the same time, EVs are heterogeneous, elusive and difficult to study due to their physical properties and the complex composition of their environment. This overview addresses the current challenges encountered when working with EVs, and how we envision that most of these challenges will be overcome in the near future. Right now, an infrastructure is being developed to improve the reproducibility of EV measurement results. This infrastructure comprises expert task forces of the International Society of Extracellular Vesicles (ISEV) developing guidelines and recommendations, instrument calibration, standardized and transparent reporting, and education. Altogether, these developments will support the credibility of EV research by introducing robust reproducibility, which is a prerequisite for understanding their biological significance and biomarker potential.
AB - Cells release membrane-delimited particles into the environment. These particles are called “extracellular vesicles” (EVs), and EVs are present in fluids contacting cells, including body fluids and conditioned culture media. Because EVs change and contribute to health and disease, EVs have become a hot topic. From the thousands of papers now published on EVs annually, one easily gets the impression that EVs provide biomarkers for all diseases, and that EVs are carriers of all relevant biomolecules and are omnipotent therapeutics. At the same time, EVs are heterogeneous, elusive and difficult to study due to their physical properties and the complex composition of their environment. This overview addresses the current challenges encountered when working with EVs, and how we envision that most of these challenges will be overcome in the near future. Right now, an infrastructure is being developed to improve the reproducibility of EV measurement results. This infrastructure comprises expert task forces of the International Society of Extracellular Vesicles (ISEV) developing guidelines and recommendations, instrument calibration, standardized and transparent reporting, and education. Altogether, these developments will support the credibility of EV research by introducing robust reproducibility, which is a prerequisite for understanding their biological significance and biomarker potential.
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U2 - 10.1016/j.ejcb.2022.151226
DO - 10.1016/j.ejcb.2022.151226
M3 - Article
C2 - 35460959
AN - SCOPUS:85128480400
SN - 0171-9335
VL - 101
JO - European journal of cell biology
JF - European journal of cell biology
IS - 3
M1 - 151226
ER -