TY - JOUR
T1 - Reporting from the field
T2 - Genetically encoded fluorescent reporters uncover signaling dynamics in living biological systems
AU - Mehta, Sohum
AU - Zhang, Jin
PY - 2011/7/7
Y1 - 2011/7/7
N2 - Real-time visualization of a wide range of biochemical processes in living systems is being made possible through the development and application of genetically encoded fluorescent reporters. These versatile biosensors have proven themselves tailor-made to the study of signal transduction, and in this review, we discuss some of the unique insights that they continue to provide regarding the spatial organization and dynamic regulation of intracellular signaling networks. In addition, we explore the more recent push to expand the scope of biological phenomena that can be monitored using these reporters, while also considering the potential to integrate this highly adaptable technology with a number of emerging techniques that may significantly broaden our view of how networks of biochemical processes shape larger biological phenomena.
AB - Real-time visualization of a wide range of biochemical processes in living systems is being made possible through the development and application of genetically encoded fluorescent reporters. These versatile biosensors have proven themselves tailor-made to the study of signal transduction, and in this review, we discuss some of the unique insights that they continue to provide regarding the spatial organization and dynamic regulation of intracellular signaling networks. In addition, we explore the more recent push to expand the scope of biological phenomena that can be monitored using these reporters, while also considering the potential to integrate this highly adaptable technology with a number of emerging techniques that may significantly broaden our view of how networks of biochemical processes shape larger biological phenomena.
KW - Spatiotemporal dynamics
UR - http://www.scopus.com/inward/record.url?scp=79959451416&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959451416&partnerID=8YFLogxK
U2 - 10.1146/annurev-biochem-060409-093259
DO - 10.1146/annurev-biochem-060409-093259
M3 - Article
C2 - 21495849
AN - SCOPUS:79959451416
SN - 0066-4154
VL - 80
SP - 375
EP - 401
JO - Annual Review of Biochemistry
JF - Annual Review of Biochemistry
ER -