Replication of RNA viruses: III. Utilization of ribonucleotide analogs in the reaction catalyzed by a RNA virus RNA polymerase

Lucille Shapiro, J. T. August

Research output: Contribution to journalArticlepeer-review

Abstract

In the reaction catalyzed by the RNA virus RNA polymerase, the ribonucleotide analogs BrUTP†, ψUTP, rTTP, BrCTP, ITP and 6-CH3 ATP each specifically replaced the comparable normal ribonucleoside triphosphate. The apparent Ks values of UTP analogs were greater for the RNA virus RNA polymerase than for the DNA-directed RNA polymerase. The data are consistent with a template mechanism utilizing hydrogen-bonded pairing of complementary bases in RNA-directed RNA synthesis. These results provide further evidence that replication of single-strand RNA viruses involves the synthesis of RNA complementary to parental RNA.

Original languageEnglish (US)
Pages (from-to)214-220
Number of pages7
JournalJournal of molecular biology
Volume14
Issue number1
DOIs
StatePublished - 1965

Keywords

  • 4-azauridine 5′-triphosphate
  • 5 bromo
  • 5-ribosyluracil 5′-triphosphate
  • 6-CH ATP
  • 6-methylamino-purine ribonucleoside triphosphate
  • AzaUTP
  • Br-
  • ITP
  • PP
  • TCA
  • XTP
  • inorganic pyrophosphate
  • inosine 5′-triphosphate
  • rRNA
  • rTTP
  • ribosomal RNA
  • ribothymidine 5′-triphosphate
  • trichloroacetic acid
  • xanthosine 5′-triphosphate
  • ψUTP

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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