Replication of RNA viruses: III. Utilization of ribonucleotide analogs in the reaction catalyzed by a RNA virus RNA polymerase

Lucille Shapiro; J. T. August

doi:10.1016/S0022-2836(65)80241-8

Replication of RNA viruses: III. Utilization of ribonucleotide analogs in the reaction catalyzed by a RNA virus RNA polymerase

Lucille Shapiro, J. T. August

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In the reaction catalyzed by the RNA virus RNA polymerase, the ribonucleotide analogs BrUTP†, ψUTP, rTTP, BrCTP, ITP and 6-CH₃ ATP each specifically replaced the comparable normal ribonucleoside triphosphate. The apparent K_s values of UTP analogs were greater for the RNA virus RNA polymerase than for the DNA-directed RNA polymerase. The data are consistent with a template mechanism utilizing hydrogen-bonded pairing of complementary bases in RNA-directed RNA synthesis. These results provide further evidence that replication of single-strand RNA viruses involves the synthesis of RNA complementary to parental RNA.

Original language	English (US)
Pages (from-to)	214-220
Number of pages	7
Journal	Journal of molecular biology
Volume	14
Issue number	1
DOIs	https://doi.org/10.1016/S0022-2836(65)80241-8
State	Published - 1965
Externally published	Yes

Keywords

4-azauridine 5′-triphosphate
5 bromo
5-ribosyluracil 5′-triphosphate
6-CH ATP
6-methylamino-purine ribonucleoside triphosphate
AzaUTP
Br-
ITP
PP
TCA
XTP
inorganic pyrophosphate
inosine 5′-triphosphate
rRNA
rTTP
ribosomal RNA
ribothymidine 5′-triphosphate
trichloroacetic acid
xanthosine 5′-triphosphate
ψUTP

ASJC Scopus subject areas

Molecular Biology
Biophysics
Structural Biology

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10.1016/S0022-2836(65)80241-8

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title = "Replication of RNA viruses: III. Utilization of ribonucleotide analogs in the reaction catalyzed by a RNA virus RNA polymerase",

abstract = "In the reaction catalyzed by the RNA virus RNA polymerase, the ribonucleotide analogs BrUTP†, ψUTP, rTTP, BrCTP, ITP and 6-CH3 ATP each specifically replaced the comparable normal ribonucleoside triphosphate. The apparent Ks values of UTP analogs were greater for the RNA virus RNA polymerase than for the DNA-directed RNA polymerase. The data are consistent with a template mechanism utilizing hydrogen-bonded pairing of complementary bases in RNA-directed RNA synthesis. These results provide further evidence that replication of single-strand RNA viruses involves the synthesis of RNA complementary to parental RNA.",

keywords = "4-azauridine 5′-triphosphate, 5 bromo, 5-ribosyluracil 5′-triphosphate, 6-CH ATP, 6-methylamino-purine ribonucleoside triphosphate, AzaUTP, Br-, ITP, PP, TCA, XTP, inorganic pyrophosphate, inosine 5′-triphosphate, rRNA, rTTP, ribosomal RNA, ribothymidine 5′-triphosphate, trichloroacetic acid, xanthosine 5′-triphosphate, ψUTP",

author = "Lucille Shapiro and August, {J. T.}",

year = "1965",

doi = "10.1016/S0022-2836(65)80241-8",

language = "English (US)",

volume = "14",

pages = "214--220",

journal = "Journal of molecular biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "1",

}

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T1 - Replication of RNA viruses

T2 - III. Utilization of ribonucleotide analogs in the reaction catalyzed by a RNA virus RNA polymerase

AU - Shapiro, Lucille

AU - August, J. T.

PY - 1965

Y1 - 1965

N2 - In the reaction catalyzed by the RNA virus RNA polymerase, the ribonucleotide analogs BrUTP†, ψUTP, rTTP, BrCTP, ITP and 6-CH3 ATP each specifically replaced the comparable normal ribonucleoside triphosphate. The apparent Ks values of UTP analogs were greater for the RNA virus RNA polymerase than for the DNA-directed RNA polymerase. The data are consistent with a template mechanism utilizing hydrogen-bonded pairing of complementary bases in RNA-directed RNA synthesis. These results provide further evidence that replication of single-strand RNA viruses involves the synthesis of RNA complementary to parental RNA.

AB - In the reaction catalyzed by the RNA virus RNA polymerase, the ribonucleotide analogs BrUTP†, ψUTP, rTTP, BrCTP, ITP and 6-CH3 ATP each specifically replaced the comparable normal ribonucleoside triphosphate. The apparent Ks values of UTP analogs were greater for the RNA virus RNA polymerase than for the DNA-directed RNA polymerase. The data are consistent with a template mechanism utilizing hydrogen-bonded pairing of complementary bases in RNA-directed RNA synthesis. These results provide further evidence that replication of single-strand RNA viruses involves the synthesis of RNA complementary to parental RNA.

KW - 4-azauridine 5′-triphosphate

KW - 5 bromo

KW - 5-ribosyluracil 5′-triphosphate

KW - 6-CH ATP

KW - 6-methylamino-purine ribonucleoside triphosphate

KW - AzaUTP

KW - Br-

KW - ITP

KW - PP

KW - TCA

KW - XTP

KW - inorganic pyrophosphate

KW - inosine 5′-triphosphate

KW - rRNA

KW - rTTP

KW - ribosomal RNA

KW - ribothymidine 5′-triphosphate

KW - trichloroacetic acid

KW - xanthosine 5′-triphosphate

KW - ψUTP

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SN - 0022-2836

VL - 14

SP - 214

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JO - Journal of molecular biology

JF - Journal of molecular biology

IS - 1

ER -

Replication of RNA viruses: III. Utilization of ribonucleotide analogs in the reaction catalyzed by a RNA virus RNA polymerase

Abstract

Keywords

ASJC Scopus subject areas

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