TY - JOUR
T1 - Replication of RNA viruses
T2 - II. The RNA product of a reaction catalyzed by a viral RNA-dependent RNA polymerase
AU - Shapiro, Lucille
AU - August, J. T.
PY - 1965/1/1
Y1 - 1965/1/1
N2 - In the reaction catalyzed by a viral RNA-dependent RNA polymerase, synthesis of RNA is directed by the RNA added to the reaction. The added RNA appears to act as template in a synthetic mechanism based on hydrogen-bonded base pairing, because the base composition of RNA synthesized in vitro is complementary to that of the RNA used to direct the reaction. When Escherichia coli W S-RNA is used as template, the RNA product of the reaction serves as a methyl group acceptor in a reaction catalyzed by methylating enzymes specific for S-RNA. The RNA product of the viral RNA polymerase reaction is partially resistant to RNase. As indicated by changes in RNase sensitivity during thermal denaturation and renaturation, the RNA product possesses a high degree of secondary structure. These results are discussed in relation to the mechanism of replication of RNA viruses.
AB - In the reaction catalyzed by a viral RNA-dependent RNA polymerase, synthesis of RNA is directed by the RNA added to the reaction. The added RNA appears to act as template in a synthetic mechanism based on hydrogen-bonded base pairing, because the base composition of RNA synthesized in vitro is complementary to that of the RNA used to direct the reaction. When Escherichia coli W S-RNA is used as template, the RNA product of the reaction serves as a methyl group acceptor in a reaction catalyzed by methylating enzymes specific for S-RNA. The RNA product of the viral RNA polymerase reaction is partially resistant to RNase. As indicated by changes in RNase sensitivity during thermal denaturation and renaturation, the RNA product possesses a high degree of secondary structure. These results are discussed in relation to the mechanism of replication of RNA viruses.
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U2 - 10.1016/S0022-2836(65)80057-2
DO - 10.1016/S0022-2836(65)80057-2
M3 - Article
C2 - 14290345
AN - SCOPUS:78651194354
SN - 0022-2836
VL - 11
SP - 272
EP - 284
JO - Journal of molecular biology
JF - Journal of molecular biology
IS - 2
ER -