Abstract
In the reaction catalyzed by the RNA virus RNA polymerase, the ribonucleotide analogs BrUTP†, ψUTP, rTTP, BrCTP, ITP and 6-CH3 ATP each specifically replaced the comparable normal ribonucleoside triphosphate. The apparent Ks values of UTP analogs were greater for the RNA virus RNA polymerase than for the DNA-directed RNA polymerase. The data are consistent with a template mechanism utilizing hydrogen-bonded pairing of complementary bases in RNA-directed RNA synthesis. These results provide further evidence that replication of single-strand RNA viruses involves the synthesis of RNA complementary to parental RNA.
Original language | English (US) |
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Pages (from-to) | 214-220 |
Number of pages | 7 |
Journal | Journal of molecular biology |
Volume | 14 |
Issue number | 1 |
DOIs | |
State | Published - 1965 |
Externally published | Yes |
Keywords
- 4-azauridine 5′-triphosphate
- 5 bromo
- 5-ribosyluracil 5′-triphosphate
- 6-CH ATP
- 6-methylamino-purine ribonucleoside triphosphate
- AzaUTP
- Br-
- ITP
- PP
- TCA
- XTP
- inorganic pyrophosphate
- inosine 5′-triphosphate
- rRNA
- rTTP
- ribosomal RNA
- ribothymidine 5′-triphosphate
- trichloroacetic acid
- xanthosine 5′-triphosphate
- ψUTP
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology