Replication of RNA bacteriophage R23. II. Inhibition of phage-specific RNA synthesis by phleomycin

Mamoru Watanabe, Thomas August

Research output: Contribution to journalArticle

Abstract

The multiplication in Escherichia coli of both DNA and RNA bacteriophages was found to be inhibited by the antibiotic phleomycin. The cessation of RNA phage replication appeared to result from inhibition of phage-directed RNA synthesis. After addition of phleomycin, there was simultaneous cessation of synthesis of both single-stranded phage RNA and RNase-resistant RNA. Synthesis of coat protein, phage assembly and the formation of infective particles were unaffected and phleomycin treatment did not result in defective particles. This drug also inhibited the synthesis of bacterial RNA, and secondarily, of bacterial protein in E. coli. Inhibition by phleomycin of viral RNA polymerase and DNA-dependent RNA polymerase activities was evident in in vitro studies and there was evidence of phleomycin binding to RNA.

Original languageEnglish (US)
Pages (from-to)21-33
Number of pages13
JournalJournal of Molecular Biology
Volume33
Issue number1
StatePublished - Apr 14 1968
Externally publishedYes

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Phleomycins
RNA Phages
DNA-Directed RNA Polymerases
Bacterial RNA
RNA
Escherichia coli
Bacterial Proteins
Viral DNA
Viral RNA
Capsid Proteins
Ribonucleases
Bacteriophages
Anti-Bacterial Agents
DNA
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Virology

Cite this

Replication of RNA bacteriophage R23. II. Inhibition of phage-specific RNA synthesis by phleomycin. / Watanabe, Mamoru; August, Thomas.

In: Journal of Molecular Biology, Vol. 33, No. 1, 14.04.1968, p. 21-33.

Research output: Contribution to journalArticle

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