Abstract
The multiplication in Escherichia coli of both DNA and RNA bacteriophages was found to be inhibited by the antibiotic phleomycin. The cessation of RNA phage replication appeared to result from inhibition of phage-directed RNA synthesis. After addition of phleomycin, there was simultaneous cessation of synthesis of both single-stranded phage RNA and RNase-resistant RNA. Synthesis of coat protein, phage assembly and the formation of infective particles were unaffected and phleomycin treatment did not result in defective particles. This drug also inhibited the synthesis of bacterial RNA, and secondarily, of bacterial protein in E. coli. Inhibition by phleomycin of viral RNA polymerase and DNA-dependent RNA polymerase activities was evident in in vitro studies and there was evidence of phleomycin binding to RNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 21-33 |
| Number of pages | 13 |
| Journal | Journal of Molecular Biology |
| Volume | 33 |
| Issue number | 1 |
| State | Published - Apr 14 1968 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
Cite this
Replication of RNA bacteriophage R23. II. Inhibition of phage-specific RNA synthesis by phleomycin. / Watanabe, Mamoru; August, Thomas.
In: Journal of Molecular Biology, Vol. 33, No. 1, 14.04.1968, p. 21-33.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Replication of RNA bacteriophage R23. II. Inhibition of phage-specific RNA synthesis by phleomycin
AU - Watanabe, Mamoru
AU - August, Thomas
PY - 1968/4/14
Y1 - 1968/4/14
N2 - The multiplication in Escherichia coli of both DNA and RNA bacteriophages was found to be inhibited by the antibiotic phleomycin. The cessation of RNA phage replication appeared to result from inhibition of phage-directed RNA synthesis. After addition of phleomycin, there was simultaneous cessation of synthesis of both single-stranded phage RNA and RNase-resistant RNA. Synthesis of coat protein, phage assembly and the formation of infective particles were unaffected and phleomycin treatment did not result in defective particles. This drug also inhibited the synthesis of bacterial RNA, and secondarily, of bacterial protein in E. coli. Inhibition by phleomycin of viral RNA polymerase and DNA-dependent RNA polymerase activities was evident in in vitro studies and there was evidence of phleomycin binding to RNA.
AB - The multiplication in Escherichia coli of both DNA and RNA bacteriophages was found to be inhibited by the antibiotic phleomycin. The cessation of RNA phage replication appeared to result from inhibition of phage-directed RNA synthesis. After addition of phleomycin, there was simultaneous cessation of synthesis of both single-stranded phage RNA and RNase-resistant RNA. Synthesis of coat protein, phage assembly and the formation of infective particles were unaffected and phleomycin treatment did not result in defective particles. This drug also inhibited the synthesis of bacterial RNA, and secondarily, of bacterial protein in E. coli. Inhibition by phleomycin of viral RNA polymerase and DNA-dependent RNA polymerase activities was evident in in vitro studies and there was evidence of phleomycin binding to RNA.
UR - http://www.scopus.com/inward/record.url?scp=0014413512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014413512&partnerID=8YFLogxK
M3 - Article
VL - 33
SP - 21
EP - 33
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -