TY - JOUR
T1 - Replication of RNA bacteriophage R23. I. Quantitative aspects of phage RNA and protein synthesis
AU - Watanabe, Mamoru
AU - Watanabe, Hiroko
AU - August, J. Thomas
N1 - Funding Information:
This work was supported in part by grants from the National Institutes of Health (GM11936 and GM11301), and the National Science Foundation (GB6082). One of us (M. W.) is a recipient of the Research Fellowship of the American College of Physicians, another (H. W.) is E recipient of the Research Fellowship of the Damon Runyon Memorial Fund for Cancer Research, and the third (J. T. A.) is a recipient of an investigatorship of the Health Research Council of the City of New York under contract 1346. This is communication no. 8’7 from the Joan and Lester Avnet Instit#ute of Molecular Biology.
PY - 1968/4/14
Y1 - 1968/4/14
N2 - Infection of Escherichia coli by R23, a newly isolated strain of RNA bacteriophage, has been found markedly to suppress synthesis of bacterial RNA. Approximately 60% of total RNA synthesized after infection was encapsulated in progeny phage particles. In contrast, after infection with the RNA phages f2 or Qβ, only 25 to 30% of newly synthesized RNA was phage RNA. Thirty to 40% of the RNA synthesized after B23 infection was characterized as a second form of phage-specific RNA by its resistance to RNase. Synthesis of bacterial proteins was also markedly inhibited after infection by R23 and ultimately replaced by phage-directed protein synthesis. B23 "early" protein synthesis, detected as phage RNA polymerase activity, was evident five to ten minutes after infection and increased until 20 to 30 minutes. In contrast, in the normally infected cell, synthesis of coat protein was predominant at a later time, beginning 30 to 45 minutes after infection and reaching a maximum between 45 and 75 minutes of infection, when coat protein constituted almost all of the newly synthesized protein. Coat protein synthesis was not detectable in the absence of RNA synthesis, whereas phage RNA polymerase induction required little or no RNA synthesis.
AB - Infection of Escherichia coli by R23, a newly isolated strain of RNA bacteriophage, has been found markedly to suppress synthesis of bacterial RNA. Approximately 60% of total RNA synthesized after infection was encapsulated in progeny phage particles. In contrast, after infection with the RNA phages f2 or Qβ, only 25 to 30% of newly synthesized RNA was phage RNA. Thirty to 40% of the RNA synthesized after B23 infection was characterized as a second form of phage-specific RNA by its resistance to RNase. Synthesis of bacterial proteins was also markedly inhibited after infection by R23 and ultimately replaced by phage-directed protein synthesis. B23 "early" protein synthesis, detected as phage RNA polymerase activity, was evident five to ten minutes after infection and increased until 20 to 30 minutes. In contrast, in the normally infected cell, synthesis of coat protein was predominant at a later time, beginning 30 to 45 minutes after infection and reaching a maximum between 45 and 75 minutes of infection, when coat protein constituted almost all of the newly synthesized protein. Coat protein synthesis was not detectable in the absence of RNA synthesis, whereas phage RNA polymerase induction required little or no RNA synthesis.
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U2 - 10.1016/0022-2836(68)90277-5
DO - 10.1016/0022-2836(68)90277-5
M3 - Article
C2 - 4869223
AN - SCOPUS:0014413527
VL - 33
SP - 1
EP - 20
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 1
ER -