TY - JOUR
T1 - Replication of proto-oncogenes early during the S phase in mammalian cell lines
AU - Iqbal, Mohammed A.
AU - Chinsky, Jeffrey
AU - Didamo, Valerie
AU - Schildkraut, Carl L.
N1 - Funding Information:
These studies were supported in part by grants to C.L.S. from the March of Dimes (1-933) the American Cancer Society (CD-239), and by the National Institutes of Health grant (GM 22332). M.A.I, was supported by a NIH training grant CA09060-09 and J.C. was supported by a NIH Program grant P01CA31855. Support from the Cancer Center Core Grant (NIH/NCI P30-CA13330) is also acknowledged. We thank Drs. Ruy Soeiro and Geoffrey
PY - 1987/1/12
Y1 - 1987/1/12
N2 - Members of several classes of proto-oncogenes replicate during the first third of S-phase in two human (K562 erythroleukemia and HeLa), one Chinese hamster (CHO) and eight mouse cell lines. These cell lines exhibit a variety of specialized functions characteristic of pre-B and B cells, T cells and erythroid cells. The proto-oncogenes studied include fos, myc, myb, abl, fes, fms, mos, raf, rel, sis, Ha-ras, Ki-ras, and N-ras. In K562 cells, amplified and rearranged c-abl genes show a pattern of temporal replication during S that is similar to the pattern observed for the 5′ breakpoint cluster region (bcr) and the amplified Cλ light chain immunoglobulin genes. The c-Ki-ras related sequences in CHO cells provide one example of late replicating proto-oncogene sequences that are present in multiple copies. The cellular gene N-myc replicates late during S in some of these cell lines. In three pre-B cell lines in which N-myc specific transcripts have been detected, N-myc replicates earlier in the S phase than in the other cell lines studied here.
AB - Members of several classes of proto-oncogenes replicate during the first third of S-phase in two human (K562 erythroleukemia and HeLa), one Chinese hamster (CHO) and eight mouse cell lines. These cell lines exhibit a variety of specialized functions characteristic of pre-B and B cells, T cells and erythroid cells. The proto-oncogenes studied include fos, myc, myb, abl, fes, fms, mos, raf, rel, sis, Ha-ras, Ki-ras, and N-ras. In K562 cells, amplified and rearranged c-abl genes show a pattern of temporal replication during S that is similar to the pattern observed for the 5′ breakpoint cluster region (bcr) and the amplified Cλ light chain immunoglobulin genes. The c-Ki-ras related sequences in CHO cells provide one example of late replicating proto-oncogene sequences that are present in multiple copies. The cellular gene N-myc replicates late during S in some of these cell lines. In three pre-B cell lines in which N-myc specific transcripts have been detected, N-myc replicates earlier in the S phase than in the other cell lines studied here.
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U2 - 10.1093/nar/15.1.87
DO - 10.1093/nar/15.1.87
M3 - Article
C2 - 3469620
AN - SCOPUS:0023103986
SN - 0305-1048
VL - 15
SP - 87
EP - 103
JO - Nucleic acids research
JF - Nucleic acids research
IS - 1
ER -