Replication in Cultured C2C12 Muscle Cells Correlates with the Neuroinvasiveness of California Serogroup Bunyaviruses

Christian Griot; Andrew Pekosz; Richard Davidson; Kara Stillmock; Maarten Hoek; David Lukac; Dan Schmeidler; Ingenue Cobbinah; Francisco Gonzalez-Scarano; Neal Nathanson

doi:10.1006/viro.1994.1308

Replication in Cultured C2C12 Muscle Cells Correlates with the Neuroinvasiveness of California Serogroup Bunyaviruses

Christian Griot, Andrew Pekosz, Richard Davidson, Kara Stillmock, Maarten Hoek, David Lukac, Dan Schmeidler, Ingenue Cobbinah, Francisco Gonzalez-Scarano, Neal Nathanson

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The neuroinvasiveness of California serogroup bunyaviruses is determined by the ability of the virus to replicate in striated muscle after peripheral inoculation of mice. Neuroinvasiveness was mapped to the medium (M) RNA segment of the virus, which encodes the viral glycoproteins, when reassortants were made between La Crosse/original virus, a neuroinvasive isolate, and Tahyna-181/57 virus, a nonneuroinvasive clone. We have tested the murine muscle cell line C2C12 as a surrogate for myotropism and have found that there is a slight, but reproducible difference in the replication of virus clones bearing the M RNA segment of La Crosse/original virus compared to clones bearing the M RNA segment of Tahyna-181/57 virus, as determined by viral titer, antigen expression, and plaque formation.

Original language	English (US)
Article number	71308
Pages (from-to)	399-403
Number of pages	5
Journal	Virology
Volume	201
Issue number	2
DOIs	https://doi.org/10.1006/viro.1994.1308
State	Published - Jun 1994
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1006/viro.1994.1308

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title = "Replication in Cultured C2C12 Muscle Cells Correlates with the Neuroinvasiveness of California Serogroup Bunyaviruses",

abstract = "The neuroinvasiveness of California serogroup bunyaviruses is determined by the ability of the virus to replicate in striated muscle after peripheral inoculation of mice. Neuroinvasiveness was mapped to the medium (M) RNA segment of the virus, which encodes the viral glycoproteins, when reassortants were made between La Crosse/original virus, a neuroinvasive isolate, and Tahyna-181/57 virus, a nonneuroinvasive clone. We have tested the murine muscle cell line C2C12 as a surrogate for myotropism and have found that there is a slight, but reproducible difference in the replication of virus clones bearing the M RNA segment of La Crosse/original virus compared to clones bearing the M RNA segment of Tahyna-181/57 virus, as determined by viral titer, antigen expression, and plaque formation.",

author = "Christian Griot and Andrew Pekosz and Richard Davidson and Kara Stillmock and Maarten Hoek and David Lukac and Dan Schmeidler and Ingenue Cobbinah and Francisco Gonzalez-Scarano and Neal Nathanson",

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doi = "10.1006/viro.1994.1308",

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AU - Griot, Christian

AU - Pekosz, Andrew

AU - Davidson, Richard

AU - Stillmock, Kara

AU - Hoek, Maarten

AU - Lukac, David

AU - Schmeidler, Dan

AU - Cobbinah, Ingenue

AU - Gonzalez-Scarano, Francisco

AU - Nathanson, Neal

PY - 1994/6

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AB - The neuroinvasiveness of California serogroup bunyaviruses is determined by the ability of the virus to replicate in striated muscle after peripheral inoculation of mice. Neuroinvasiveness was mapped to the medium (M) RNA segment of the virus, which encodes the viral glycoproteins, when reassortants were made between La Crosse/original virus, a neuroinvasive isolate, and Tahyna-181/57 virus, a nonneuroinvasive clone. We have tested the murine muscle cell line C2C12 as a surrogate for myotropism and have found that there is a slight, but reproducible difference in the replication of virus clones bearing the M RNA segment of La Crosse/original virus compared to clones bearing the M RNA segment of Tahyna-181/57 virus, as determined by viral titer, antigen expression, and plaque formation.

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Replication in Cultured C2C12 Muscle Cells Correlates with the Neuroinvasiveness of California Serogroup Bunyaviruses

Abstract

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