Replication-defective adenovirus serotype 5 vectors elicit durable cellular and humoral immune responses in nonhuman primates

Sampa Santra, Michael S. Seaman, Ling Xu, Dan H. Barouch, Carol I. Lord, Michelle A. Lifton, Darci A. Gorgone, Kristin R. Beaudry, Krisha Svehla, Brent Welcher, Bimal K. Chakrabarti, Yue Huang, Zhi Yong Yang, John R. Mascola, Gary J. Nabel, Norman L. Letvin

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Abstract

The magnitude and durability of immune responses induced by replication-defective adenovirus serotype 5 (ADV5) vector-based vaccines were evaluated in the simian-human immunodeficiency virus/rhesus monkey model. A single inoculation of recombinant ADV5 vector constructs induced cellular and humoral immunity, but the rapid generation of neutralizing anti-Ad5 antibodies limited the immunity induced by repeated vector administration. The magnitude and durability of the immune responses elicited by these vaccines were greater when they were delivered as boosting immunogens in plasmid DNA-primed monkeys than when they were used as single-modality immunogens. Therefore, administration of ADV5-based vectors in DNA-primed subjects may be a preferred use of this vaccine modality for generating long-term immune protection.

Original languageEnglish (US)
Pages (from-to)6516-6522
Number of pages7
JournalJournal of Virology
Volume79
Issue number10
DOIs
Publication statusPublished - May 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology

Cite this

Santra, S., Seaman, M. S., Xu, L., Barouch, D. H., Lord, C. I., Lifton, M. A., ... Letvin, N. L. (2005). Replication-defective adenovirus serotype 5 vectors elicit durable cellular and humoral immune responses in nonhuman primates. Journal of Virology, 79(10), 6516-6522. https://doi.org/10.1128/JVI.79.10.6516-6522.2005