Reorganization of actin filaments enhances chondrogenic differentiation of cells derived from murine embryonic stem cells

Zijun Zhang, Joseph Messana, Nathaniel S H Hwang, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Differentiation of embryonic stem cells is of great interest to developmental biology and regenerative medicine. This study investigated the effects of cytochalasin D (CD) on the distribution of actin filaments in mouse embryoid body (EB)-derived cells. Furthermore, CD was applied to chondrogenic medium to examine its chondrogenic effect. CD at a concentration of 1 μg/ml disrupted stress fibers in EB-derived cells. Actin filaments in treated cells reorganized into a peripheral pattern, and type II collagen was detected by immunocytochemistry. The expression of type II collagen, Sox9, and at a later time point, aggrecan was up-regulated after CD treatment. In the CD-treated cells, Oct4 and Sox2, representing undifferentiation, were down-regulated as well as Sox1, AFP, and CTN-1, representing ectoderm, endoderm, and cardiogenesis, respectively. In conclusion, CD treatment enhances chondrogenesis of EB-derived cells. Moreover, it promotes a more complete stem cell differentiation toward chondrogenesis, when cultured in chondrogenic medium.

Original languageEnglish (US)
Pages (from-to)421-427
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume348
Issue number2
DOIs
StatePublished - Sep 22 2006

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Keywords

  • Actin
  • Chondrogenesis
  • Cytoskeleton
  • Differentiation
  • Embryonic stem cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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